AI Article Synopsis
- The study examined how Fe(II) in a citrate solution affects DNA strand scission while considering the role of (-)-epigallocatechin gallate (EGCg).
- The rate of oxygen consumption by Fe(II) correlated with the DNA damage, and the presence of EGCg was found to speed up these reactions.
- The research demonstrated that EGCg can inhibit DNA damage by scavenging harmful hydroxyl radicals, which could help in assessing antioxidant activity against these radicals.
Article Abstract
The DNA strand scission induced by Fe(II) in a citrate buffer solution and the effect of (-)-epigallocatechin gallate (EGCg) were kinetically analyzed. The rate of consumption of dissolved oxygen by Fe(II) in each of these solutions was measured and paralleled that DNA scission. Coordinated EGCg accelerated these reactions. Curves of the time-course characteristics of DNA scission were simulated by using the rate constant of oxygen consumption and by assuming that scission with the hydroxyl radical (OH), which was formed from the dissolved oxygen, proceeded competitively with the scavenging of OH by citrate, Cl- ions and EGCg added. Free EGCg acted as a DNA scission inhibitor to scavenge OH, in contrast to the case of the coordinated one. This analysis is useful for estimating the rate constant of the reaction between an antioxidant and OH, and might provide a new method for measuring the OH-scavenging activity.
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| http://dx.doi.org/10.1271/bbb.66.770 | DOI Listing |
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