AI Article Synopsis
- Researchers examined the brains of 5 BSE-affected cattle and 3 scrapie-affected sheep, comparing them to normal brains using a histoblot technique.
- The histoblot technique allowed for the specific detection of normal (PrP(C)) and abnormal (PrP(Sc)) prion protein isoforms.
- In the brains affected by BSE or scrapie, there was a noticeable decrease in the PrP(C) signal, particularly in areas showing the presence of the PrP(Sc) signal.
Article Abstract
To detect prion protein, brains from 5 cattle naturally affected with bovine spongiform encephalopathy (BSE) and 3 sheep naturally affected with scrapie were examined and compared with brains of normal cattle and sheep using a histoblot technique. The technique enabled the in situ distinctive detection of the cellular (PrP(C)) and abnormal (PrP(Sc)) isoforms of the prion protein. In BSE- or scrapie-affected brains, the Prp(C) signal decreased, especially in those areas where the PrP(Sc) signal was detected.
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| http://dx.doi.org/10.1177/104063870201400313 | DOI Listing |
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