A new HLA-DR12 allele has been identified in a European Caucasoid bone marrow donor. The DRB1*12012 allele differs from DRB1*12011 by two silent substitutions at codons 72 and 78, two polymorphic positions used for DNA subtyping of the DR12 serotype. The co-occurence of the two nucleotide changes is unique to the DR12 group and results in a new PCR-SSP typing pattern. The complete HLA type of the donor is A24, A68; B55, B61; Cw*01, Cw*0304; DRB1*12012, DRB1*1402; DRB3*0101, DRB3*0202; DQB1*0301. HLA-DRB1*12012 is a rare allele as it occurs in < 0.2% of DR12 donors.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1034/j.1399-0039.2002.590221.x | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Association analysis of T and B-cell epitopes with humoral alloimmunisation in kidney transplantation: A Tunisian cohort study.
Hum Immunol
January 2025
Immunology department, Hedi Chaker Hospital, University of Sfax, Sfax, Tunisia.
Introduction: HLA matching is critical for successful kidney transplantation. This study aimed to investigate the impact of eplet mismatches and Predicted Indirectly Recognizable HLA Epitopes (PIRCHE-II) scores on the development of de novo donor-specific antibodies (dnDSA) and graft survival in a Tunisian cohort, characterized by a high prevalence of living donors and significant genetic diversity in HLA profiles.
Methods: This retrospective study included 112 adult kidney transplant recipients who underwent transplantation between 2012 and 2018.
[Study on the Production of Anti-D and Anti-E Mixed Antibodies by Alloimmunization in RhD Variant Type33 Recipients].
Zhongguo Shi Yan Xue Ye Xue Za Zhi
December 2024
Blood Group Reference Laboratory, Ningxia Blood Center, Yinchuan 750000, Ningxia Hui Autonomous Region, China.
Objective: To investigate the cause of the production of anti-D and anti-E mixed antibody in an RhD positive patient.
Methods: The ABO/Rh blood group typing and irregular antibody specificity were identified by conventional serological methods, the gene exon 1-10 and heterozygous analysis were performed by sequence-specific primer polymerase chain reaction (PCR-SSP), and the whole exon sequence was analyzed by first-generation sequencing.
Results: The patient's Rh blood group was weak D Type33, with the allele was , the patients was found to be heterozygous, with an Rh typing of Ccee, and the patient had developed anti-D combined with anti-E mixed antibodies.
HLA class I and class II alleles and haplotypes of Algerian population from Algiers and neighbouring area.
Transfus Clin Biol
November 2024
Immunology Department, Mustapha Bacha University Hospital, Algiers, Algeria; University of Health Sciences, Algiers, Algeria.
In this study, we aimed to investigate the current genetic diversity and provide additional insights into the origins of the Algerian population by analyzing the frequencies of HLA -A,-B,-DRB1,-DQB1 alleles and associated haplotypes. We analyzed 1,082 unrelated healthy Algerian individuals, who were potential kidney donors, recruited and assessed in the Immunology Department of CHU Mustapha in Algiers over a 10-year period (2009-2019). HLA genotyping was performed by Polymerase Chain Reaction Sequence Specific Primers (PCR-SSP).
View Article and Find Full Text PDFPitfalls When Determining HNA-1 Genotypes and Finding Novel Alleles.
Int J Mol Sci
August 2024
Department of Clinical Immunology, Aalborg University Hospital, 9000 Aalborg, Denmark.
Genetic variation in the gene is responsible for different variants of human neutrophil antigen 1 (HNA-1). Laboratory techniques currently utilized for routine HNA-1 genotyping, predominantly PCR-sequence-specific primer (PCR-SSP) and PCR-sequence-based typing (PCR-SBT), lack specificity for . This study compares the capabilities and limitations of existing technologies including an in-house TaqMan PCR, a commercial PCR-SSP test, PCR-SBT and multiplex ligation-dependent probe amplification (MLPA) with those of a long-read nanopore sequencing assay.
View Article and Find Full Text PDFDetecting serologically difficult ABO blood groups using single-molecule real-time sequencing technology.
Vox Sang
October 2024
Department of Blood Transfusion, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui, China.
Background And Objectives: Recently, third-generation long-read sequencing technology has been increasingly applied to the detection of various blood group systems. Because of its long read length and use of single-molecule sequencing, it is capable of obtaining the sequences of blood group genes in their entirety as well as of distinguishing haplotypes. Therefore, here, we collected ABO blood group samples that were difficult to classify serologically and analysed the sequences of the coding regions of the ABO genes as well as the sequences upstream and downstream of the coding regions.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!