Objective: To construct a statistical model for the agreements and disagreements between two observers on darkness of staining.
Study Design: Data from an earlier observer-agreement study by van Diest et al were reanalyzed.
Results: A model in which the random variation in error is permitted to depend upon the true darkness of staining wasfound tofit the data much better than does one in which the random variation is constant.
Conclusion: For the dataset analyzed, error tends to be greater (that is, correlation between observers tends to be less) when staining is darker.
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ACS Chem Neurosci
January 2025
Department of Physiology, Faculty of Medicine, Firat University, Elazığ 23200, Türkiye.
This study evaluates acetylcholinesterase (AChE) enzyme activity levels, oxidative stress parameters, histopathological findings, and serum melatonin levels in rat brain tissue. 32 male Wistar Albino rats were randomly divided into four groups: Control, Light, Dark, Dim light ( = 8 each group). After a 30 day experiment, brain tissues were collected to measure AChE, glutathione S-transferase (GST), glutathione (GSH), and malondialdehyde (MDA) levels and conduct histopathological analyses.
View Article and Find Full Text PDFInt J Mol Sci
December 2024
Department of Clinical Biochemistry, Bispebjerg University Hospital, 2400 Copenhagen, Denmark.
Many endocrine glands exhibit circadian rhythmicity, but the interplay between the central circadian clock in the suprachiasmatic nucleus (SCN), the peripheral endocrine clock, and hormones is sparsely understood. We therefore studied the cellular localizations of the clock protein PER1, parathyroid hormone (PTH) and calcitonin (CT) in the parathyroid and thyroid glands, respectively. Thyroid glands, including the parathyroids, were dissected at different time-points from rats housed in 12 h:12 h light-darkness cycles, and were double-immunostained for PER1 and PTH or CT.
View Article and Find Full Text PDFHarmful Algae
August 2024
CAS Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences Qingdao, 266071 China; Laboratory for Marine Ecology and Environmental Science, Qingdao Marine Science and Technology Center, Qingdao 266237, China; Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao 266071, China. Electronic address:
Coral reef ecosystems are the most productive and biodiverse marine ecosystems, with their productivity levels highly dependent on the symbiotic dinoflagellates belonging to the family Symbiodiniaceae. As a unique life history strategy, resting cyst production is of great significance in the ecology of many dinoflagellate species, those HABs-causing species in particular, however, there has been no confirmative evidence for the resting cyst production in any species of the family Symbiodiniaceae. Based on morphological and life history observations of cultures in the laboratory and morpho-molecular detections of cysts from the marine sediments via fluorescence in situ hybridization (FISH), cyst photography, and subsequent singe-cyst PCR sequencing, here we provide evidences for the asexual production of resting cysts by Effrenium voratum, the free-living, red tide-forming, and the type species of the genus Effrenium in Symbiodiniaceae.
View Article and Find Full Text PDFPhys Chem Chem Phys
June 2024
Department of Physical Chemistry 1, Lund University, SE-22100 Lund, Sweden.
Neurosurg Rev
April 2024
Department of Neurosurgery, University of São Paulo, São Paulo, SP, Brazil.
The treatment for peripheral nerve sheath tumors (PNSTs) is based on surgical excision and the primary goal is to improve symptoms whilst preserving neurological function. In order to improve this technique, surgeons may use sodium fluorescein (SF) to help visualize the neoplasm and, consequently, facilitate its removal. Aiming to assess the efficacy of this emerging surgical strategy, we conducted a systematic review and single-arm meta-analysis.
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