The use of cyclic voltammetric measurements for the determination of kinetic constants in enzyme-based electrocatalysis. The case of the redox mediated dihydronicotinamide adenine dinucleotide oxidation catalyzed by flavoproteins.

On the basis of simulated voltammetric curves used as testing data, practical methods to extract the bimolecular rate constant for a mediated enzymatic catalysis with an excess of the substrate are examined. The evaluation criteria are the simplicity of treatment and the agreement between the expected and the obtained values of the rate constants. The results obtained indicate that the different approaches examined are equivalent.