A simple method for measuring the hydrophobicity of bacterial cells is proposed, which is based on the spectrophotometric evaluation of cell adherence to chloroform drops in a biphasic water-chloroform mixture.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Dendritic polyglutamic acid-chelerythrine nanocomplex for the reversal of bacterial tooth decay.
J Mater Chem B
March 2025
College of Polymer Science and Engineering, State Key Laboratory of Polymer Materials Engineering, Sichuan University, Chengdu 610065, China.
Caries begin with an imbalance between demineralization and remineralization due to the continuous acid production by cariogenic bacteria. However, the development of dental materials that could prevent and treat caries a simple and efficient mechanism has always been a challenge. To address this issue, therapeutic nanoparticles composed of a dendritic polyglutamic acid (DPGlu) and chelerythrine (CHE) complex (DPGlu@CHE) were developed hydrophobic interactions and hydrogen bonds.
View Article and Find Full Text PDFSystematic All-Hydrocarbon Stapling Analysis for Cecropin A Generates a Potent and Stable Antimicrobial Peptide.
J Med Chem
March 2025
School of Pharmacy, Second Military Medical University, Shanghai 200433, China.
As an evolutionarily conserved family of antimicrobial peptides (AMPs), cecropins play an important role in innate immunity. But their inevitable weaknesses, including poor proteolytic stability and unpredictable cytotoxicity, severely hindered their clinical applications. Considering their two-helical structure, all-hydrocarbon stapling was performed on cecropin A, successfully generating 27 (, + 4) stapled derivatives.
View Article and Find Full Text PDFNanocoating of quinoa protein and hyaluronic acid enhances viability and stability of Limosilactobacillus fermentum RC4 microcapsules.
Int J Biol Macromol
March 2025
State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Ningbo University, Ningbo, Zhejiang 315211, China; Key Laboratory of Animal Protein Food Processing Technology of Zhejiang Province, College of Food and Pharmaceutical Sciences, Ningbo University, Ningbo 315800, China; Zhejiang-Malaysia Joint Research Laboratory for Agricultural Product Processing and Nutrition, Ningbo 315800, China.
Nanocoating represents an effective strategy for creating a protective barrier on probiotic surfaces, preventing them from damage. Here, we developed HAQ microcapsules comprising Limosilactobacillus fermentum RC4, which were nanocoated with hyaluronic acid and quinoa protein. We characterized the stability and safety, and investigated the intermolecular forces and transcriptome to elucidate the mechanisms underlying the nanocoating.
View Article and Find Full Text PDFThe ABC transporter MsbA in a dozen environments.
Structure
February 2025
Osnabrück University, Department of Biology/Chemistry, Structural Biology Section, 49076 Osnabrück, Germany; Center of Cellular Nanoanalytics Osnabrück (CellNanOs), 49076 Osnabrück, Germany. Electronic address:
High-resolution structure determination of membrane proteins typically requires reconstitution into artificial membrane mimics. The choice of the specific membrane substitute can strongly affect the protein's specific activity, stability, and conformational spectrum, potentially leading to errors or misinterpretation during analysis. The bacterial ATP-binding cassette transporter MsbA is a prominent example of such environment-specific bias.
View Article and Find Full Text PDFNano-formulated curcumin uptake and biodistribution in the fetal growth restricted newborn piglet brain.
Drug Deliv Transl Res
March 2025
Department of Chemical Engineering, University of Washington, Seattle, WA, USA.
Fetal growth restriction (FGR) affects 5% to 10% of all pregnancies in developed countries and is the second most leading cause of perinatal mortality and morbidity. Life-long consequences of FGR range from learning and behavioral issues to cerebral palsy. To support the newborn brain following FGR, timely and accessible neuroprotection strategies are needed.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!