This study describes a method to detect in vivo Pigeon circovirus (PiCV, also called Columbid circovirus, CoCV) identified recently. Blood samples of healthy and diseased pigeons were investigated. DNA was isolated from a drop of blood spotted and dried on filter paper. A nested PCR using two primer pairs from the cap gene was performed to detect PiCV. Using this method, PiCV nucleic acid was detected in 17 of 53 pigeons examined. PCR using DNA from dried blood samples is a simple, fast and reliable technique that allows detection of PiCV in vivo and facilitates collection, storage and shipment of samples.
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http://dx.doi.org/10.1016/s0166-0934(02)00038-1 | DOI Listing |
Pathogens
January 2025
Department of Veterinary Science, Faculty of Agriculture, University of Miyazaki, Miyazaki 889-2192, Japan.
Viruses in the family can infect mammals and birds. Porcine circovirus type 2 (PCV2) significantly affects the livestock industry by causing porcine circovirus-associated diseases, such as postweaning multisystem wasting syndrome, respiratory disease complex, and dermatitis nephropathy syndrome. Additionally, beak and feather disease virus in parrots, canine circovirus in dogs, and columbid circovirus (pigeon circovirus) in racing pigeons induce immunosuppression, followed by secondary infections in these hosts.
View Article and Find Full Text PDFMicrobiol Resour Announc
October 2024
Swine and Poultry Infectious Diseases Research Center (CRIPA-FRQ), Faculté de médecine vétérinaire, Université de Montréal, Saint-Hyacinthe, Québec, Canada.
A necropsy was performed on a Common Raven () presenting an opportunistic fungal respiratory infection and a bursal lymphoid depletion with inclusion bodies, suggestive of a circovirus infection. High-throughput sequencing of circular DNA in the bursa of Fabricius revealed a complete genome sequence of a strain.
View Article and Find Full Text PDFSci Rep
June 2024
Biodesign Center for Fundamental and Applied Microbiomics, Center for Evolution and Medicine, School of Life Sciences, Arizona State University, Tempe, USA.
This study was aimed to investigate the frequency of PiCV recombination, the kinetics of PiCV viremia and shedding and the correlation between viral replication and host immune response in young pigeons subclinically infected with various PiCV variants and kept under conditions mimicking the OLR system. Fifteen racing pigeons originating from five breeding facilities were housed together for six weeks. Blood and cloacal swab samples were collected from birds every seven days to recover complete PiCV genomes and determine PiCV genetic diversity and recombination dynamics, as well as to assess virus shedding rate, level of viremia, expression of selected genes and level of anti-PiCV antibodies.
View Article and Find Full Text PDFVet Res Forum
March 2024
Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
An internationally recognized syndrome that leads to deaths among domestic and ornamental pigeons, particularly after racing, is young pigeon disease syndrome (YPDS). Pigeon circovirus (PiCV) is regarded as one of the potential factors contributing to the occurrence of YPDS. This survey was conducted to determine the prevalence of PiCV infection and molecularly characterize the PiCV in pigeons suspected of YPDS.
View Article and Find Full Text PDFBMC Genomics
April 2024
National Laboratory Animal Quality Testing Center, National Institutes for Food and Drug Control, Beijing, 102629, People's Republic of China.
Background: Pigeon circovirus infections in pigeons (Columba livia domestica) have been reported worldwide. Pigeons should be PiCV-free when utilized as qualified experimental animals. However, pigeons can be freely purchased as experimental animals without any clear guidelines to follow.
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