A new method for fixing Biomphalaria glabrata for histologic studies, using shell perforation.

Mem Inst Oswaldo Cruz

Departamento de Parasitologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brasil.

Published: March 2002

A new technique for fixation of Biomphalaria glabrata for histologic studies is described. It consists in performing several external holes in the shell, before placing the entire snail into the fixative. It is a very practical and quick procedure that showed excellent results when compared to the usual techniques.

Download full-text PDF

Source
http://dx.doi.org/10.1590/s0074-02762002000200024DOI Listing

Publication Analysis

Top Keywords

biomphalaria glabrata
8
glabrata histologic
8
histologic studies
8
method fixing
4
fixing biomphalaria
4
studies shell
4
shell perforation
4
perforation technique
4
technique fixation
4
fixation biomphalaria
4

Similar Publications

Schistosomiasis, a neglected tropical disease, is transmitted by freshwater snails. Interruption of transmission will require novel vector-focused interventions. We performed a genome-wide association study of African snails, , exposed to in an endemic area of high transmission in Kenya.

View Article and Find Full Text PDF

Genomic basis of schistosome resistance in a molluscan vector of human schistosomiasis.

iScience

January 2025

Program in Public Health, College of Health Sciences, University of California, Irvine, Irvine, CA 92697, USA.

Freshwater snails are obligate intermediate hosts for the transmission of schistosomiasis, one of the world's most devastating parasitic diseases. To decipher the mechanisms underlying snail resistance to schistosomes, recombinant inbred lines (RILs) were developed from two well-defined homozygous lines (iM line and iBS90) of the snail . Whole-genome sequencing (WGS) was used to scan the genomes of 46 individual RIL snails, representing 46 RILs, half of which were resistant or susceptible to .

View Article and Find Full Text PDF

Iron oxide nanoparticles (IONPs) are prominent in nanomedicine, cosmetics, and environmental applications. However, their increasing production, use, and release into the environment raises concerns about their potential risks to aquatic life and human health. This study aimed to evaluate the bioaccumulation, as concentration of small, medium and large iron aggregates in the digestive tubules, histopathological changes, and inflammatory responses in the freshwater snail Biomphalaria glabrata following chronic exposure to gluconic-acid functionalized IONPs (GLA-IONPs) compared to their dissolved counterpart (FeCl).

View Article and Find Full Text PDF

Lake Victoria is a well-known hot spot for intestinal schistosomiasis, caused by infection with the trematode Schistosoma mansoni. The snail intermediate hosts of this parasite are Biomphalaria snails, with Biomphalaria choanomphala being the predominant intermediate host within Lake Victoria. The prevalence of S.

View Article and Find Full Text PDF

Schistosomiasis is a neglected tropical disease of public health significance. In view of its elimination as a public health problem by 2030, adopting a One Health approach is necessary, considering its multidimensional nature. Animal reservoirs, in particular, pose a significant threat to schistosomiasis control in Africa and beyond.

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!