The optimal conditions for the formation of a stable mixed urease/octadecylamine (ODA) film at the air-water interface are determined. This film is efficiently transferred onto the hydrophobized surface of a pH-ISFET, and the features of the urea enzymatic field-effect transistor (ENFET) are determined: detection limit 0.2 mM, response time 15 s, and dynamic range 0-20 mM.
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http://dx.doi.org/10.1016/s1567-5394(02)00045-2 | DOI Listing |
Bioelectrochemistry
May 2002
IFOS-LPC1, UMR CNRS 5621, Ecole Centrale de Lyon, 36 Avenue Guy de Collongue, BP 163, F69131 Ecully Cedex, France.
The optimal conditions for the formation of a stable mixed urease/octadecylamine (ODA) film at the air-water interface are determined. This film is efficiently transferred onto the hydrophobized surface of a pH-ISFET, and the features of the urea enzymatic field-effect transistor (ENFET) are determined: detection limit 0.2 mM, response time 15 s, and dynamic range 0-20 mM.
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