The specific structural features of stem-loop (hairpin) DNA constructs provide increased specificity of target recognition. Recently, several robust assays have been developed that exploit the potential of structurally constrained oligonucleotides to hybridize with their cognate targets. Here, I review new diagnostic approaches based on the formation of stem-loop DNA oligonucleotides: molecular beacon methodology, suppression PCR approaches and the use of hairpin probes in DNA microarrays. The advantages of these techniques over existing ones for sequence-specific DNA detection, amplification and manipulation are discussed.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/s0167-7799(02)01942-x | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Screening and application of aptamers as fluorescent biosensors for selective and sensitive detection of hepatocellular carcinoma and in vivo targeted delivery studies.
Mikrochim Acta
October 2024
School of Chemistry & Chemical Engineering, Inner Mongolia University, Hohhot, 010020, China.
The incidence of primary hepatocellular carcinoma (HCC) has recently ranked fifth in the world, and the incidence rate is increasing year by year worldwide. Therefore, early diagnosis is the highest priority in the treatment of HCC. In this paper, four anti-HCC aptamers were obtained using magnetic bead SELEX technology.
View Article and Find Full Text PDFDual dye-labeled aptamers for detection of dichlorvos using ratiometric fluorescence resonance energy transfer.
Anal Chim Acta
November 2024
State Key Laboratory of Toxicology and Medical Countermeasures, Laboratory of Toxicant Analysis, Academy of Military Medical Sciences, Beijing, 100850, China. Electronic address:
Article Synopsis
- Dichlorvos (DDVP) is a highly toxic pesticide, making the monitoring of its residues in the environment and human health crucial, but traditional detection methods are cumbersome and not suitable for on-site testing.
- The study involved the development and testing of two aptamer probes, finding that DV55 was more effective than DV65 for detecting DDVP, with a fast reaction time of just 5 minutes and a detection range of 0-100 μM.
- The research highlights the potential of these dual-labeled aptamer probes as a portable, efficient method for rapid on-site detection of DDVP, showing promising results for future applications in pesticide monitoring.
A compact stem-loop DNA aptamer targets a uracil-binding pocket in the SARS-CoV-2 nucleocapsid RNA-binding domain.
Nucleic Acids Res
November 2024
Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis, MN 55455, USA.
SARS-CoV-2 nucleocapsid (N) protein is a structural component of the virus with essential roles in the replication and packaging of the viral RNA genome. The N protein is also an important target of COVID-19 antigen tests and a promising vaccine candidate along with the spike protein. Here, we report a compact stem-loop DNA aptamer that binds tightly to the N-terminal RNA-binding domain of SARS-CoV-2 N protein.
View Article and Find Full Text PDFAmplification-free nucleic acids detection with next-generation CRISPR/dx systems.
Crit Rev Biotechnol
September 2024
Department of Biology, College of Science, Shantou University, Shantou, Guangdong, China.
CRISPR-based diagnostics (CRISPR/Dx) have revolutionized the field of molecular diagnostics. It enables home self-test, field-deployable, and point-of-care testing (POCT). Despite the great potential of CRISPR/Dx in diagnoses of biologically complex diseases, preamplification of the template often is required for the sensitive detection of low-abundance nucleic acids.
View Article and Find Full Text PDFPeptide nucleic acids can form hairpins and bind RNA-binding proteins.
PLoS One
September 2024
Currently or formerly at School of Life and Environmental Sciences, The University of Sydney, Sydney, NSW, Australia.
RNA-binding proteins (RBPs) are a major class of proteins that interact with RNAs to change their fate or function. RBPs and the ribonucleoprotein complexes they constitute are involved in many essential cellular processes. In many cases, the molecular details of RBP:RNA interactions differ between viruses, prokaryotes and eukaryotes, making prokaryotic and viral RBPs good potential drug targets.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!