AI Article Synopsis
- The study aimed to explore the expression levels of HIF-2alpha/EPAS1 in bladder cancer.
- Two specific bladder cancer cell lines were analyzed for HIF-2alpha/EPAS1 mRNA and protein levels, revealing notable expression.
- The findings suggest that HIF-2alpha/EPAS1 is primarily present in invasive forms of bladder cancer, indicating its potential role in cancer invasion.
Article Abstract
Objectives: To investigate the expression of hypoxia-inducible factor (HIF)-2alpha/endothelial PAS domain protein 1 (EPAS1) in bladder cancer.
Methods: Two bladder cancer cell lines (T24, EJ-1) were examined for the expression of HIF-2alpha/EPAS1 mRNA by reverse transcriptase-polymerase chain reaction and HIF-2alpha/EPAS1 protein by Western blot analysis, respectively. Expression of HIF-2alpha/EPAS1 protein was also investigated immunohistochemically on paraffin-embedded sections from 24 patients with bladder cancer.
Results: HIF-2alpha/EPAS1 mRNA and protein could be detected in the two bladder cell lines under normoxia. The normal urothelium and all 7 superficial cases (pTis-pTa) were negative for HIF-2alpha/EPAS1. Five of the 8 pT1 cases (62.5%) and all 9 muscle-invasive cases were positive. The invasive compartment showed stronger HIF-2alpha/EPAS1 immunoreactivity compared with the superficial compartment. All the tumor clusters infiltrating to the interstitial tissue among muscle fibers showed moderate to strong positive staining.
Conclusions: HIF-2alpha/EPAS1 is mostly expressed in invasive bladder cancer, which indicates a possible role for HIF-2alpha/EPAS1 in the invasion of bladder cancer.
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| http://dx.doi.org/10.1016/s0090-4295(01)01607-7 | DOI Listing |
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