AI Article Synopsis
Article Abstract
We describe the cloning and characterization of a novel member of the immunoglobulin superfamily, Igsf9. The predicted protein structure of IGSF9 closely matches that of the neural cell-adhesion molecule (NCAM) subfamily, consisting of an extracellular region containing five immunoglobulin domains and two fibronectin type III (FnIII) repeats, a transmembrane region, and a cytoplasmic tail. We have also characterized the orthologous human IGSF9 gene at 1q22-q23, revealing a highly conserved sequence and genomic organization. Expression of Igsf9 was detected by RT-PCR in mouse embryonic RNA from embryonic day (E) 7.5 to E16.5, while whole-mount in situ hybridization at E10.5 shows intense expression within the dorsal root ganglia, trigeminal ganglia, and olfactory epithelium, and less intense expression in the neuroepithelium, retina, and hindgut. In the human, transcription was detected in a wide variety of fetal tissues at both 8 and 14 weeks. Protein homology of IGSF9 is most similar to the Drosophila melanogaster Turtle protein that functions in coordinated motor output in complex behaviors.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1006/geno.2002.6757 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Expression, purification and characterization of phosphatidylserine-targeting antibodies for biochemical and therapeutic applications.
Methods Cell Biol
January 2025
Department of Microbiology, Biochemistry and Molecular Genetics, (3)Center for Cell Signaling, Rutgers New Jersey Medical School, Newark, NJ, United States. Electronic address:
The externalization of Phosphatidylserine (PS) from the inner surface of the plasma membrane to the outer surface of the plasma membrane is an emblematic event during apoptosis and serves as a potent "eat-me" signal for the efferocytosis of apoptotic cells. Although less well understood, PS is also externalized on live cells in the tumor microenvironment and on live virus-infected cells whereby it serves as an immune modulatory signal that drives tolerance and immune escape. Given the importance of PS in cancer immunology and immune escape, PS-targeting monoclonal antibodies have been characterized with promising immunotherapeutic potential.
View Article and Find Full Text PDFStructure and catalytic activity of a dihydrofolate reductase-like enzyme from Leptospira interrogans.
Int J Biol Macromol
January 2025
Center of Excellence for Molecular Biology and Genomics of Shrimp, Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand; Center of Excellence in Molecular Crop, Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand. Electronic address:
A dihydrofolate reductase (DHFR)-like enzyme from Leptospira interrogans (LiDHFRL) was cloned and the recombinant protein was characterized. Sequence alignment suggested that the enzyme lacked the conserved catalytic residues found in DHFR. Indeed, LiDHFRL did not catalyze the reduction of dihydrofolate by either NADH or NADPH.
View Article and Find Full Text PDFInvestigating Subpopulation Dynamics in Clonal CHO-K1 Cells with Single-Cell RNA Sequencing.
J Biotechnol
January 2025
Johns Hopkins Biomedical Engineering; Johns Hopkins University Department of Molecular Biology and Genetics, Baltimore, Maryland, USA; Johns Hopkins University Department of Medicine, Division of Infectious Disease, Baltimore, Maryland, USA. Electronic address:
Chinese Hamster Ovary (CHO) cells produce monoclonal antibodies and other biotherapeutics at industrial scale. Despite their ubiquitous nature in the biopharmaceutical industry, little is known about the behaviors of individual transfected clonal CHO cells. Most CHO cells are assessed on their stability, their ability to produce the protein of interest over time.
View Article and Find Full Text PDFCloning and functional verification of gene in .
PeerJ
January 2025
College of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan, Shandong, China.
Background: Geraniol 10-hydroxylase (G10H) is a cytochrome P450 monooxygenase involved in regulation, which is involved in the biosynthesis of monoterpene. However, G10H is not characterized at the enzymatic mechanism and regulatory function in .
Methods And Results: A gene related to the biosynthesis of monoterpenoid, geraniol 10-hydroxylase, has been cloned from the medicinal plant .
Neuroendocrine prostate cancer drivers SOX2 and BRN2 confer differential responses to imipridones ONC201, ONC206, and ONC212 in prostate cancer cell lines.
Am J Transl Res
December 2024
Laboratory of Translational Oncology and Experimental Cancer Therapeutics, The Warren Alpert Medical School, Brown University Providence, RI 02903, USA.
Objectives: Prostate cancer (PCa) is a leading cause of cancer death in men worldwide. Approximately 30% of castrate-resistant PCa becomes refractory to therapy due to neuroendocrine differentiation (NED) that is present in <1% of de-novo tumors. First-in-class imipridone ONC201/TIC10 therapy has shown clinical activity against midline gliomas, neuroendocrine tumors, and PCa.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!