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Poly(L-lysine)-based cylindrical copolypeptide brushes as potential drug and gene carriers.
J Control Release
September 2015
Medical College, Shantou University, Shantou 515041, China. Electronic address:
Histidinylated poly-L-lysine-based vectors for cancer-specific gene expression via enhancing the endosomal escape.
J Biomater Sci Polym Ed
October 2014
a Graduate School of Systems Life Sciences , Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395 , Japan.
In this work, we synthesized a series of poly-L-lysine (PLL)-based polymers for gene delivery, by modifying the PLL with both cationic peptide and histidine. The peptide moieties serve as cationic centers for polyplex formation, and also as substrates for protein kinase Cα (PKCα), which is specifically activated in many types of cancer cells, to achieve cancer-specific gene expression. The histidine groups serve as buffering moieties to increase the ability of the plasmid DNA (pDNA)-polymer complex (polyplex) to escape the endosome and thus to promote expression of the pDNA in the transfected cells.
View Article and Find Full Text PDFsiRNA-based therapy ameliorates glomerulonephritis.
J Am Soc Nephrol
April 2010
Department of Nephrology and Endocrinology, University of Tokyo Graduate School of Medicine, 7-3-1, Hongo, Bunkyo-ku, Tokyo 113-8655, Japan.
RNA interference by short interfering RNAs (siRNAs) holds promise as a therapeutic strategy, but use of siRNAs in vivo remains limited. Here, we developed a system to target delivery of siRNAs to glomeruli via poly(ethylene glycol)-poly(l-lysine)-based vehicles. The siRNA/nanocarrier complex was approximately 10 to 20 nm in diameter, a size that would allow it to move across the fenestrated endothelium to access to the mesangium.
View Article and Find Full Text PDFBiomaterial-mediated retroviral gene transfer using self-assembled monolayers.
Biomaterials
December 2007
Coulter Department of Biomedical Engineering, Georgia Institute of Technology, Emory University, Atlanta, GA 30332, USA.
Biomaterial-mediated gene delivery has recently emerged as a promising alternative to conventional gene transfer technologies that focus on direct delivery of viral vectors or DNA-polymer/matrix complexes. However, biomaterial-based strategies have primarily targeted transient gene expression vehicles, including plasmid DNA and adenovirus particles. This study expands on this work by characterizing biomaterial properties conducive to the surface immobilization of retroviral particles and subsequent transduction of mammalian cells at the cell-material interface.
View Article and Find Full Text PDFPoly-L-lysine-based gene delivery systems. Synthesis, purification, and application.
Methods Mol Med
January 2003
Target Protein Technologies, San Diego, CA, USA.
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