We examined if axon terminals of dopaminergic neurons might make synapses upon neurokinin-1 receptor (NK1R)-expressing intrinsic neurons in the rat striatum. In a double-immunocytochemical ultrastructural study, dopaminergic terminals were labeled by the immunoperoxidase method for tyrosine hydroxylase (TH), while NK1R-immunoreactivity (-IR) was revealed by the immunogold-silver labeling method. Some TH-immunoreactive (-ir) axon terminals formed synapses of the symmetric or intermediate type on NK1R-ir neuronal profiles; usually on dendritic profiles and rarely on somatic profiles. It was further confirmed by means of triple-immunofluorescence histochemistry that NK1R-ir neurons in close association with TH-ir axon terminals showed nitric oxide synthase (NOS)- or vesicular acetylcholine transporter-IR. Since NK1R-expressing striatal neurons are segregated into cholinergic and somatostatin/NOS-containing intrinsic neurons (Brain Res. 631 (1993) 297; Neurosci. Lett. 310 (2001) 109), the present results indicate that dopaminergic neurons make synapses upon these intrinsic neurons in the striatum.
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