[Molecular markers in the epidemiologic study of Legionella pneumophila infections].

Purpose: Legionnaires' disease is due to the inhalation of contaminated aerosols. The identification of the source of contamination in the aquatic environment is necessary to prevent the occurrence of new cases. A comparative study of clinical and environmental isolates is the basis of epidemiological investigations.

Current Knowledge And Key Points: Genotypic methods are now mainly used to compare bacterial strains. Some of these methods are based on the electrophoretic separation of DNA restriction fragments. When electrophoretic profile are complex, some fragments can be visualized after hybridization (ribotyping). Large-sized fragments can be separated by pulsed-field gel electrophoresis. Other techniques are based on gene amplification, such as AP-PCR. This technique is easy to perform but its discriminatory power and reproducibility are lower. Some procedures are combining enzymatic cleavage and gene amplification. Finally methods based on the nucleotide sequence analysis of some genes are being evaluated.

Future Prospects And Projects: Techniques enabling the rapid comparison of various Legionella isolates will permit a quick detection of outbreaks and contribute to the identification of the source of contamination.