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[Investigation of the effect of various buffer solutions used for microinjection of gene-engineering constructs on preimplantation development of mouse embryos]. | LitMetric

AI Article Synopsis

  • The study investigated the impact of various buffer solutions on the development of mouse embryos after microinjection in zygotes.
  • Buffers such as Tris-HCl with EDTA, Tris-HCl with MgCl2, NaCl, and medium M2 showed a significantly lower survival rate of embryos reaching the blastocyst stage (p < 0.05).
  • No significant differences were observed in survival rates among zygotes injected with different buffer solutions.

Article Abstract

We studied the effects of some buffer solutions used for microinjection in mammalian zygotes on preimplantation development of (CBA x C57BL)F1 mouse embryos in vitro. The rate of embryo survival was estimated according to their capacity to develop to the stages of blastocyst and blastocyst hatched from zona pellucida. The results obtained suggested a reduced rate of survival of zygotes to the blastocyst stage after the injection into a pronucleus of the buffers Tris-HCl with EDTA, Tris-HCl with MgCl2 and NaCl, and medium M2 (p < 0.05) and to the stage of blastocyst hatched from zone pellucida after injection of a Dulbecco solution, as compared to the control. No differences were found in the survival rate of zygotes injected with different buffer solutions.

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