Vitamin C is present in the cytosol as ascorbic acid, functioning primarily as a cofactor for enzymatic reactions and as an antioxidant to scavenge free radicals. Human granulocyte macrophage-colony-stimulating factor (GM-CSF) induces an increase in reactive oxygen species (ROS) and uses ROS for some signaling functions. We therefore investigated the effect of vitamin C on GM-CSF-mediated responses. Loading U937 cells with vitamin C decreased intracellular levels of ROS and inhibited the production of ROS induced by GM-CSF. Vitamin C suppressed GM-CSF-dependent phosphorylation of the signal transducer and activator of transcription 5 (Stat-5) and mitogen-activated protein (MAP) kinase (Erk1 and Erk2) in a dose-dependent manner as was phosphorylation of MAP kinase induced by both interleukin 3 (IL-3) and GM-CSF in HL-60 cells. In 293T cells transfected with alpha and beta GM-CSF receptor subunits (alphaGMR and betaGMR), GM-CSF-induced phosphorylation of betaGMR and Jak-2 activation was suppressed by vitamin C loading. GM-CSF-mediated transcriptional activation of a luciferase reporter construct containing STAT-binding sites was also inhibited by vitamin C. These results substantiate the importance of ROS in GM-CSF signaling and indicate a role for vitamin C in downmodulating GM-CSF signaling responses. Our findings point to vitamin C as a regulator of cytokine redox-signal transduction in host defense cells and a possible role in controlling inflammatory responses.
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http://dx.doi.org/10.1182/blood.v99.9.3205 | DOI Listing |
Plant Foods Hum Nutr
January 2025
Centro Interdisciplinario de Investigaciones en Tecnologías y Desarrollo Social para el NOA (CIITED), Facultad de Ingeniería, Universidad Nacional de Jujuy and Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Jujuy, Argentina.
The mineral content, vitamin C, and chlorogenic acid were determined in 44 genotypes of Andean potatoes reintroduced in the Quebrada de Humahuaca, Jujuy, Argentina. This initiative aims to promote biodiversity and support local producers by determining the nutritional and functional properties of these genotypes. The genotypes from a collection in the Germplasm Bank of INTA Balcarce, were planted in sandy clay loam soils and harvested manually.
View Article and Find Full Text PDFTrop Anim Health Prod
January 2025
College of Animal Sciences, Anhui Science and Technology University, Fengyang, 233100, China.
This study was aim to investigate the effects of lipoic acid (ALA) on performance, meat quality, serum biochemistry and antioxidant function of broilers under heat stress (HS). Two hundred1-day-old Cobb broilers were randomly divided into four treatment groups and each treatment consisted of 4 replicates of 10 broilers each. The treatment group adopts a 2 × 2 two-factor setting, which is divided into two diets (basic diet or 250 mg/kg ALA diet) and two temperatures (24 ± 1℃ or 33 ± 1℃).
View Article and Find Full Text PDFBiol Aujourdhui
January 2025
Institut d'Écologie et des Sciences de l'Environnement de Paris (iEES Paris), Paris, France - Sorbonne Université, 4 place Jussieu, 75005 Paris, France.
The evolutionary success of angiosperms, which make up more than 95 percent of the world's terrestrial flora, is largely based on their interactions with animal pollinators. Indeed, it is estimated that, on average, 87.5 percent of flowering plants are pollinated by animals.
View Article and Find Full Text PDFJ Sci Food Agric
January 2025
School of Biosciences, Faculty of Science and Engineering, University of Nottingham Malaysia, Semenyih, Malaysia.
In recent years, changes in dietary patterns from an omnivore diet to a moderate-to-restrictive diet that includes more plant food are becoming popular for various reasons and the associated health benefits. Despite the increased consumption of plant food as recommended by these seemingly healthy diets, micronutrient deficiency is still prevalent particularly among the health-conscious populations. The aim of this review is to help guide interventions by understanding micronutrient deficiency trends from a dietary habit and plant physiology context.
View Article and Find Full Text PDFEndocrinol Diabetes Metab Case Rep
January 2025
Summary: Oral levothyroxine (LT4) is prescribed worldwide for hypothyroidism. Bariatric surgery for patients with obesity has shown a substantial, long-term weight loss and considerable improvement of obesity-related diseases. LT4 malabsorption represents a significant cause of refractory hypothyroidism, well known after malabsorptive bariatric surgery such as Roux-en-Y gastric bypass.
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