pH and CO2 content differences between fish(Cyprinus carpio) gills micro-environment and the ambient environment were investigated using the technique developed by Playle. The effect of the pH change on elutriation of copper sorbed on gibbsite was also studied in view of possible desorption of the metal within the gill micro-environment. It was found that there was no pH difference around pH 8.0 and 0.2-0.4 unit difference was observed between the gills' inspired and the expired water when the ambient pH varied from 8 up to 9.5 or from 8 down to 7.0. Similar trend was detected for CO2. More Cu adsorbed on the gibbsite can be removed by the acidified expired water from the fish gill micro-environment, comparing to the inspired water.
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