The required HLB values of eucalyptus, lippia and peppermint essential oils were determined using droplet size analysis and turbidimetric method on emulsions prepared with emulsifier blends of varying HLB values. The percentage increase in mean droplet diameter and the degree of dispersion of droplet sizes were determined before and after centrifugation of the emulsions. The HLB value of the emulsion with the least dispersion ratio or the least percentage increase in mean droplet diameter was taken as the required HLB of the respective essential oil. The turbidimetric method was validated by the existence of correlation (r=-0.958) between the mean droplet diameter and the turbidity of the emulsions. The turbidity curve went through a maximum at the HLB value where the mean droplet diameter was least. Based on these methods, the required HLB values of eucalyptus, lippia and peppermint oils were determined as 9.8, 12.1 and 12.3, respectively (P<0.05). Liquid paraffin was used as a reference standard and its required HLB fell within literature value.
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