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[Study on expression of cell cycle-related genes in subclonal cell lines of human cervical carcinoma]. | LitMetric

Objective: To explore the role of expression of cell cycle-related genes in cervical carcinoma cell lines.

Methods: A series of expression microarray analysis of two homologous cervical carcinoma subclonal cell lines were initiated by cDNA microarray which represent a set of 234 human cell cycle-related genes.

Results: In normal medium, the percent of G(1) phase in CS03 cells was higher than in CS07 cells dramatically, but the percent of S phase in CS07 cells was more than in CS03 cells. After cultured 48 h in serum-free medium, the percent of apoptosis cells (sub-G(1) phase) in CS03 cells was higher than in CS07 cells significantly and increased with time. By applying this cDNA microarray, we identified 3 differentially expressed genes in two homologous cell lines, which were BN51, hsp90 and Mcl-1 genes, further identified 3 upregulated genes in CS07 cell line, the ratio of Cy5/Cy3 was 0.480, 0.479 and 0.490 respectively.

Conclusion: Differential expression of BN51, hsp90 and Mcl-1 genes is associated to various growth patterns of human cervical carcinoma cells.

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