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Chromosome transfer via cell fusion.
Methods Mol Biol
July 2011
Istituto di Ricerca Genetica e Biomedica, Consiglio Nazionale delle Ricerche, Monserrato, Italy.
Intact chromosomes as well as chromosome fragments can be vehicled into various recipient cells without perturbing their ability to segregate as free elements; chromosome transfer can be performed both in cultured cells and in living animals. The method of choice to shuttle single chromosomes between cells is microcell fusion named microcell mediated chromosome transfer (MMCT). The use of MMCT is mandatory in a number of applications where alternative chromosome transfection procedures are ineffective; however, the main drawback is the extremely low efficiency of the technique.
View Article and Find Full Text PDFConstruction of somatic cell hybrids.
Curr Protoc Hum Genet
May 2001
Rhode Island Hospital and Brown University, Providence, Rhode Island, USA.
Somatic cell hybridization is the method of choice to separate a chromosome of interest from the full chromosome complement and obtain a permanent source of the chromosome. This unit begins with the choice of fusion techniques and selectable markers for hybrids containing a chromosome of interest. The first set of protocols outline the production of whole-cell hybrids by fusion of two cell lines: a monolayer (adherent) recipient and a donor that may be adherent or grown in suspension.
View Article and Find Full Text PDFGenetics of cellular senescence.
Mech Ageing Dev
April 2002
Sam and Ann Barshop Center for Longevity and Aging Studies, University of Texas Health Science Center at San Antonio, STCBM, 15355 Lambda Drive, San Antonio, TX 78245-3207, USA.
Cellular senescence or replicative senescence is a state of irreversible growth arrest that somatic cells enter as a result of replicative exhaustion. This can be mimicked by culture manipulations such as Ras oncogene overexpression or treatment with various agents such as sodium butyrate and 5-azacytidine. It is believed that cellular senescence is one of the protective mechanisms against tumor formation.
View Article and Find Full Text PDFWhole-cell and microcell fusion for the identification of natural regulators of telomerase.
Methods Mol Biol
October 2002
Shire BioChem Inc., Laval, Quebec, Canada.
TSLC1 is a tumor-suppressor gene in human non-small-cell lung cancer.
Nat Genet
April 2001
Tumor Suppression & Functional Genomics Project, National Cancer Center Research Institute, Tokyo, Japan.
The existence of tumor-suppressor genes was originally demonstrated by functional complementation through whole-cell and microcell fusion. Transfer of chromosome 11 into a human non-small-cell lung cancer (NSCLC) cell line, A549, suppresses tumorigenicity. Loss of heterozygosity (LOH) on the long arm of chromosome 11 has been reported in NSCLC and other cancers.
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