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Modulation of the Oxygenation State and Intracellular pH of Erythrocytes by Inositol-Trispyrophosphate Investigated by P NMR Study of 2,3-DPG.
J Cell Mol Med
January 2025
Centre for Molecular Biophysics, UPR CNRS 4301, Orleans, France.
The hypoxic microenvironment is crucial for tumour cell growth and invasiveness. Tumour tissue results from adaptation to reduced oxygen availability. Hypoxia first activates pro-angiogenic signals for alleviation.
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- Quantitative Nuclear Magnetic Resonance (qNMR), specifically H-qNMR, helps determine the purity of organic molecules, but complexity in signal identification can be challenging.
- Researchers focused on the simpler phosphorus (P) nucleus for a new P-qNMR method using organophosphorus drugs like cyclophosphamide and sofosbuvir.
- They found that using aprotic solvents like DMSO-d improved accuracy in measuring purities, and P-qNMR gave results consistent with H-qNMR, leading to a validated and reproducible P-qNMR method.
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- P-NMR is easier to analyze than H-NMR, making it simpler to identify target signals for quantitation.
- The study presents a method for determining the purity of brigatinib (BR), an organophosphorus compound, using quantitative P-NMR (P-qNMR) across multiple labs.
- Results showed that the purity of BR was 97.94 ± 0.69% with P-qNMR, closely matching the 97.26 ± 0.71% found using H-qNMR, suggesting P-qNMR is a viable alternative for measuring organophosphorus compounds.
Mutations to the BTN2A1 Linker Region Impact Its Homodimerization and Its Cytoplasmic Interaction with Phospho-Antigen-Bound BTN3A1.
J Immunol
July 2023
Department of Pharmaceutical Sciences, University of Connecticut, Storrs, CT.
Intracellular binding of small-molecule phospho-Ags to the HMBPP receptor complex in infected cells leads to extracellular detection by T cells expressing the Vγ9Vδ2 TCR, a noncanonical method of Ag detection. The butyrophilin proteins BTN2A1 and BTN3A1 are part of the complex; however, their precise roles are unclear. We suspected that BTN2A1 and BTN3A1 form a tetrameric (dimer of dimers) structure, and we wanted to probe the importance of the BTN2A1 homodimer.
View Article and Find Full Text PDFCombined Use of MALDI-TOF Mass Spectrometry and P NMR Spectroscopy for the Analysis of (Phospho)Lipids.
Methods Mol Biol
January 2023
Faculty of Medicine, Institute for Medical Physics and Biophysics, Leipzig University, Leipzig, Germany.
Lipids are important and abundant constituents of all biological tissues and body fluids. In particular, phospholipids (PLs) constitute a major part of the cellular membrane and play a role in signal transduction, and some selected PLs are increasingly considered as potential disease markers. Unfortunately, methods of lipid analysis are less established in comparison to techniques of protein analysis.
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