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[Approaches for improving L-asparaginase expression in heterologous systems].
Biomed Khim
February 2023
Institute of Biomedical Chemistry, Moscow, Russia.
L-asparaginase (EC 3.5.1.
View Article and Find Full Text PDFDiscovery and Engineering of the Cocaine Biosynthetic Pathway.
J Am Chem Soc
December 2022
State Key Laboratory of Phytochemistry and Plant Resources in West China, and CAS Center for Excellence in Molecular Plant Sciences, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China.
Cocaine, the archetypal tropane alkaloid from the plant genus , has recently been used clinically as a topical anesthesia of the mucous membranes. Despite this, the key biosynthetic step of the requisite tropane skeleton (methylecgonone) from the identified intermediate 4-(1-methyl-2-pyrrolidinyl)-3-oxobutanoic acid (MPOA) has remained, until this point, unknown. Herein, we identify two missing enzymes (CYP81AN15 and MT4) necessary for the biosynthesis of the tropane skeleton in cocaine by transient expression of the candidate genes in .
View Article and Find Full Text PDFPlant cell cultures as heterologous bio-factories for secondary metabolite production.
Plant Commun
September 2021
Max-Planck-Institut für Molekulare Pflanzenphysiologie, Am Mühlenberg 1, 14476 Potsdam-Golm, Germany.
Synthetic biology has been developing rapidly in the last decade and is attracting increasing attention from many plant biologists. The production of high-value plant-specific secondary metabolites is, however, limited mostly to microbes. This is potentially problematic because of incorrect post-translational modification of proteins and differences in protein micro-compartmentalization, substrate availability, chaperone availability, product toxicity, and cytochrome p450 reductase enzymes.
View Article and Find Full Text PDFPTEN Expression Regulates Gap Junction Connectivity in the Retina.
Front Neuroanat
May 2021
Department of Neurobiology, David Geffen School of Medicine at Los Angeles, University of California, Los Angeles, Los Angeles, CA, United States.
Manipulation of the phosphatase and tensin homolog (PTEN) pathway has been suggested as a therapeutic approach to treat or prevent vision loss due to retinal disease. In this study, we investigated the effects of deleting one copy of in a well-characterized class of retinal ganglion cells called α-ganglion cells in the mouse retina. In retinas, α-ganglion cells did not exhibit major changes in their dendritic structure, although most cells developed a few, unusual loop-forming dendrites.
View Article and Find Full Text PDFDistinction between peanut allergy and tolerance by characterization of B cell receptor repertoires.
Allergy
September 2021
Center for Translational Immunology, University Medical Center Utrecht, Utrecht University, Utrecht, The Netherlands.
Background: Specific IgE against a peanut 2S albumin (Ara h 2 or 6) is the best predictor of clinically relevant peanut sensitization. However, sIgE levels of peanut allergic and those of peanut sensitized but tolerant patients partly overlap, highlighting the need for improved diagnostics to prevent incorrect diagnosis and consequently unnecessary food restrictions. Thus, we sought to explore differences in V(D)J gene transcripts coding for peanut 2S albumin-specific monoclonal antibodies (mAbs) from allergic and sensitized but tolerant donors.
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