Endonuclease of restriction of type II has been found in Bacillus subtilis B-5044 during testing of endophytic strains of cotton plants bacilli. The restrictase Bsu5044I hydrolysed DNA of M13mp18 phage in 4 sites; pUC19 DNA in 5 sites; pBR322 in 15 sites. There were a lot of sites restriction in DNAs of phages T7 and lambda. A comparison of electrophoretical divisions of Bsu5044I and Cfr13I fragments of phages and plasmid DNAs showed their identity. Thus, the found restrictase Bsu5044I is an isoschisomer of AsuI.
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