Analysis of gene expression in rose petals using expressed sequence tags.

FEBS Lett

Reproduction et Développement des Plantes, UMR 5667 CNRS-INRA-ENSL, Ecole Normale Supérieure de Lyon, 46 allée d'Italie, 69364 Cedex 07, Lyon, France.

Published: March 2002

AI Article Synopsis

  • Single-pass sequences from 1794 rose petal cDNA clones were analyzed, revealing 242 sequence groups and 635 single entries, totaling 877 distinct genes.
  • Researchers assigned potential functions to 1151 of the identified transcripts, with some showing specific expression in petals and stamens.
  • This cDNA library and database provide a significant resource for future studies aimed at enhancing important rose traits like flower shape, longevity, and fragrance.

Article Abstract

Single-pass sequences were obtained from the 5'-ends of a total of 1794 rose petal cDNA clones. Cluster analysis identified 242 groups of sequences and 635 singletons indicating that the database represents a total of 877 genes. Putative functions could be assigned to 1151 of the transcripts. Expression analysis indicated that transcripts of several of the genes identified accumulated specifically in petals and stamens. The cDNA library and expressed sequence tag database described here represent a valuable resource for future research aimed at improving economically important rose characteristics such as flower form, longevity and scent.

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http://dx.doi.org/10.1016/s0014-5793(02)02413-4DOI Listing

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