Background: It is well known that telomerase activity is expressed in cancer cells. A recent study has revealed that weak telomerase activity was also detected in normal cells. Telomerase activity is thus thought to be useful as a diagnostic biomarker. In the present study, we studied the difference in telomerase activity between cancer tissue and noncancerous epithelium tissue of the esophagus.
Methods: One hundred and thirty specimens of esophageal cancer tissue were collected at surgery and analyzed in a telomeric repeat amplification protocol (TRAP) assay. Telomerase was expressed quantitatively as the total product generated (TPG) value.
Results: Telomerase activity was detected in all esophageal cancer tissues and in 87% of the noncancerous epithelium specimens. The mean TPG values in the cancer tissue and the noncancerous epithelium specimens were 49.5 +/- 39.5 (mean +/- 1 SD) and 11.5 +/- 5.9, respectively, with a significant difference (P < 0.0001).
Conclusion: The telomerase activity in esophageal cancers was significantly higher than that in the noncancerous epithelium. From the mean value plus 1 SD for noncancerous epithelium, the cutoff value was set at 17.4 as an esophageal cancer-specific diagnostic marker. This cutoff value showed a high predictive value, specificity, and sensitivity, of greater than 80% for each category. These results suggest that this cutoff value could be a useful biomarker for the detection of esophageal carcinoma.
Download full-text PDF |
Source |
---|---|
http://dx.doi.org/10.1007/s101470200003 | DOI Listing |
Int J Mol Sci
January 2025
A.N. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 119991 Moscow, Russia.
Apurinic/apyrimidinic (AP) sites are endogenous DNA lesions widespread in human cells. Having no nucleobases, they are noncoding and promutagenic. AP site repair is generally initiated through strand incision by AP endonuclease 1 (APE1).
View Article and Find Full Text PDFRSC Med Chem
December 2024
Department of Pharmaceutical Chemistry, College of Pharmacy, The University of Mashreq Baghdad 10023 Iraq.
Many cancers have displayed resistance to chemotherapeutic drugs over the past few decades. EGFR has emerged as a leading target for cancer therapy inhibiting tumor angiogenesis. Besides, studies strongly suggest that blocking telomerase activity could be an effective way to control the growth of certain cancer cells.
View Article and Find Full Text PDFSci Rep
January 2025
Department of Thoracic Surgery, The First Affiliated Hospital, Fujian Medical University, Fuzhou, 350005, China.
Non-small cell lung cancer (NSCLC), half of which are lung adenocarcinoma (LUAD), is one of the most widely spread cancers in the world. Telomerase, which maintains telomere length and chromosomal integrity, enables cancer cells to avoid replicative senescence. When telomerase is inhibited, cancer cells' senescence began, preventing them from growing indefinitely.
View Article and Find Full Text PDFLife Sci
January 2025
Division of Applied Life Science (BK21 Four), Gyeongsang National University, Jinju 52828, Gyeongnam Province, Republic of Korea; Institute of Agriculture and Life Science, Gyeongsang National University, Gyeongnam Province, Republic of Korea; Division of Animal Science, Gyeongsang National University, Jinju 52828, Republic of Korea; The King Kong Corp. Ltd., Gyeongsang National University, Jinju 52828, Gyeongnam Province, Republic of Korea. Electronic address:
Telomerase is highly expressed in oocyte cumulus cells and plays a significant role in follicular development and oocyte maturation. In this study, we hypothesized that in vitro culture conditions may affect telomerase activity during in vitro embryo production (IVP) and that its activation may improve embryo quality. We first examined telomerase protein levels and localization in bovine cumulus-oocyte complexes via immunofluorescence assays.
View Article and Find Full Text PDFTher Adv Rare Dis
January 2025
Department of Pediatrics, Hospital Nacional Edgardo Rebagliati Martins, Lima, Peru.
Hemophagocytic lymphohistiocytosis (HLH) is a life-threatening systemic hyperinflammatory syndrome, rarely associated with bone marrow failure (BMF). Telomere biology disorders (TBD) are caused by inherited defects in telomerase processes and can have heterogeneous presentations including idiopathic pulmonary fibrosis, cirrhosis, and BMF. We report a case of a 10-year-old male from Lima, Peru, who presented with HLH as the initial manifestation of a TBD.
View Article and Find Full Text PDFEnter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!