An alkalophilic strain of Penicillium sp. RR 99 was isolated that was found to synthesise extra-cellular alpha-amylase and xylanase, when cultivated in presence of starch and xylan respectively. The strain showed maximum alpha-amylolytic activity on 4th day and maximum xylanolytic activity on 6th day of cultivation. The ability of the strain to hydrolyse starchy and hemicellulosic wastes made the strain competent not only for the commercial production of these enzymes but also for successful utilization of wastes.
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Front Nutr
December 2024
College of Animal Science, Shanxi Agricultural University, Taigu, Shanxi, China.
Background: The use of enzymes within pig feed can reduce the challenges associated with antibiotic-free animal feeding. However, this enzymatic effect is often limited by the internal and external gut environment. This study aimed to improve diet quality and assess the impact of an enzymatically hydrolyzed diet (EHD) on growth performance, meat quality, and intestinal health in growing pigs.
View Article and Find Full Text PDFMicrob Cell Fact
December 2024
Swammerdam Institute for Life Sciences, University of Amsterdam, Science Park 904, C3.108, Amsterdam, 1098 XH, The Netherlands.
Background: Bacillus subtilis is widely used for industrial enzyme production due to its capacity to efficiently secrete proteins. However, secretion efficiency of enzymes varies widely, and optimizing secretion is crucial to make production commercially viable. Previously, we have shown that overexpression of the xylanase XynA lowers expression of Clp protein chaperones, and that inactivation of CtsR, which regulates and represses clp transcription, increases the production of XynA.
View Article and Find Full Text PDFFood Chem X
December 2024
Department of Food Science and Agricultural Chemistry, Macdonald Campus, McGill University, 21,111 Lakeshore, St-Anne de Belle Vue, Québec H9X 3V9, Canada.
PLoS One
October 2024
Laboratory for Genetics and Biochemistry of Microorganisms, National Center for Biotechnology, Astana, Kazakhstan.
Keratinases, a subclass of proteases, are used to degrade keratin thereby forming peptones and free amino acids. Bacillus paralicheniformis strain T7 was isolated from soil and exhibited high keratinase, protease, collagenase, amylase, xylanase, lipase, and phosphatase activities. Keratinases of the strain showed maximum activity at 70°C and pH 9.
View Article and Find Full Text PDFMicrob Cell Fact
September 2024
Department of Chemical and Biomolecular Engineering, BK21 Plus Program, KAIST, 291 Daehak-ro, Yuseong-gu, Daejeon, 34141, Republic of Korea.
Background: Corynebacterium glutamicum is an attractive host for secretory production of recombinant proteins, including high-value industrial enzymes and therapeutic proteins. The choice of an appropriate signaling peptide is crucial for efficient protein secretion. However, due to the limited availability of signal peptides in C.
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