A full length cDNA clone encoding plastidic fructose-1,6-bisphosphatase (cp-FBPase), together with a transit peptide, was isolated from a potato (Solanum tuberosum L.) leaf cDNA library. Potato plants were transformed with the isolated cp-FBPase sequence behind a patatin class I promoter to ensure tuber-specific expression of the enzyme. Plant lines were selected which expressed up to 250 mU (g FW)-1 in the developing tubers, which is 10- to 20-fold the activity found in wild-type tubers. Intact amyloplasts were isolated from in vitro-grown minitubers developed in darkness. Comparison with marker enzymes showed that cp-FBPase activity in transgenic tubers, as well as the low FBPase activity in the wild-type tubers, was localised inside the amyloplasts. The intact amyloplasts isolated from both wild-type and transgenic tubers synthesised starch from [U-14C] glucose-6-phosphate. Conversely, only the transgenic tubers expressing cp-FBPase showed appreciable synthesis of starch from [U-14C] dihydroxyacetone phosphate, and this synthesis rate was correlated to the activity of cp-FBPase. Thus, the expression of cp-FBPase in tubers allows for a new route of starch biosynthesis from triose-phosphates imported from the cytosol. The transgenic tubers did not differ from wild-type tubers with respect to starch content, or the levels of neutral sugars and phosphorylated hexoses.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/s004250100647 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Effects of two amino acid transporter-like genes on potato growth.
J Plant Physiol
January 2025
Huzhou Wuxing Jinnong Ecological Agriculture Development Co. LTD, Huzhou, Zhejiang, 313000, People's Republic of China. Electronic address:
Amino acid transporters are membrane proteins that mediate amino acid transport across the plasma membrane. They play a significant role in plant growth and development. The amino acid permease (AAP) subfamily belongs to the activating transcription factor family, which is one of the main amino acid transporter families.
View Article and Find Full Text PDFUbiquitin Ligase Positively Regulates Drought Stress in Potato ( L.).
Int J Mol Sci
December 2024
College of Life Science and Technology, Gansu Agricultural University, Lanzhou 730070, China.
The ubiquitin-proteasome system (UPS) is a key protein degradation pathway in eukaryotes, in which E3 ubiquitin ligases mediate protein ubiquitination, directly or indirectly targeting substrate proteins to regulate various biological processes, including plant growth, hormone signaling, immune responses, and adaptation to abiotic stress. In this study, we identified plant U-box protein 51 in () as an E3 ubiquitin ligase through transcriptomic analysis, and used it as a candidate gene for gene-function analysis. Quantitative real-time PCR (qRT-PCR) was used to examine expression across different tissues, and its expression patterns under simulated drought stress induced by polyethylene glycol (PEG 6000) were assessed.
View Article and Find Full Text PDFOverexpression of the Potato Gene Enhances Drought Resistance and Root Development in Transgenic Plants.
Int J Mol Sci
November 2024
State Key Laboratory of Aridland Crop Science, College of Agronomy, Gansu Agricultural University, Lanzhou 730070, China.
Article Synopsis
- Drought significantly limits potato growth, and PYL proteins are vital for plants' response to drought stress.
- Researchers identified a specific potato gene that is highly expressed in tubers and up-regulated during drought and ABA stress.
- Functional studies showed that this gene improves drought resistance in transgenic tobacco, enhancing plant growth and root development while reducing damage from stress.
Refining polyploid breeding in sweet potato through allele dosage enhancement.
Nat Plants
January 2025
Crops Research Institute, Guangdong Academy of Agricultural Sciences and Key Laboratory of Crop Genetic Improvement of Guangdong Province, Guangzhou, China.
Article Synopsis
- The study focuses on how allele dosage affects variations in traits of hexaploid sweet potato, based on deep sequencing of 294 accessions, creating a genome-wide variation map.* -
- Genome-wide association studies revealed quantitative trait loci that link allele dosage to 23 agronomic traits, highlighting how sweet potato breeding has selectively increased these alleles to improve crop performance.* -
- The research uncovers the evolutionary trend in the Mesoamerican gene pool towards higher dosage of beneficial alleles, with evidence from transgenic validation and identification of sequence variations influencing traits like tuber weight and flesh color.*
Distinct effects of PTST2b and MRC on starch granule morphogenesis in potato tubers.
Plant Biotechnol J
February 2025
John Innes Centre, Norwich Research Park, Norwich, NR4 7UH, UK.
The molecular mechanisms underpinning the formation of the large, ellipsoidal starch granules of potato tuber are poorly understood. Here, we demonstrate the distinct effects of PROTEIN TARGETING TO STARCH2b (PTST2b) and MYOSIN RESEMBLING CHLOROPLAST PROTEIN (MRC) on tuber starch granule morphology. A gene duplication event in the Solanaceae resulted in two PTST2 paralogs (PTST2a and PTST2b).
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!