AI Article Synopsis
- The study analyzed the mouse brain proteome, revealing 8,767 proteins and identifying 1,324 polymorphic proteins through advanced techniques like two-dimensional electrophoresis and mass spectrometry.
- 665 of these proteins were genetically mapped, highlighting that many variations in proteins are due to changes in their conformation and mass.
- The findings suggest that some proteins may act as polygenic traits, with implications for understanding the genetic factors relevant to diseases.
Article Abstract
Proteome analysis is a fundamental step in systematic functional genomics. Here we have resolved 8,767 proteins from the mouse brain proteome by large-gel two-dimensional electrophoresis. We detected 1,324 polymorphic proteins from the European collaborative interspecific backcross. Of these, we mapped 665 proteins genetically and identified 466 proteins by mass spectrometry. Qualitatively polymorphic proteins, to 96%, reflect changes in conformation and/or mass. Quantitatively polymorphic proteins show a high frequency (73%) of allele-specific transmission in codominant heterozygotes. Variations in protein isoforms and protein quantity often mapped to chromosomal positions different from that of the structural gene, indicating that single proteins may act as polygenic traits. Genetic analysis of proteomes may detect the types of polymorphism that are most relevant in disease-association studies.
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