Inhibition of interleukin-6-activated janus kinases/signal transducers and activators of transcription but not mitogen-activated protein kinase signaling in liver of endotoxin-treated rats.

Objectives: Endotoxin-induced cytokines, such as interleukin-6, mediate systemic inflammatory responses through multiple cellular signaling pathways. Interleukin-6 is also responsible for the synthesis of acute phase proteins. Recent studies have shown that endotoxin can inhibit signal transducers and activators of transcription (STAT)-3 tyrosine phosphorylation in cultured cells, suggesting that this effect may limit the synthesis of acute phase proteins. The purpose of this study was to examine the effects of endotoxin on interleukin-6 activation of STATs and mitogen-activated protein (MAP) kinase pathways in rat liver in vivo.

Design: Controlled laboratory study.

Setting: Medical school laboratory.

Subjects: Specific pathogen-free male Sprague Dawley rats.

Interventions: Under anesthesia, interleukin-6 was injected into the portal vein of rats 4 hrs after the bolus intravenous administration of endotoxin (1 mg/kg) or saline. The effects of interleukin-6 on key intermediates in early steps of the interleukin-6 signaling pathway, including janus kinase-1, gp 130, the interleukin-6 receptor, STAT1, and STAT3, were examined in both saline and endotoxin-treated rats.

Measurements And Main Results: In endotoxin-treated rats, there was significant inhibition of interleukin-6 activation of janus kinase-1, gp 130, the interleukin-6 receptor, STAT1, and STAT3. These signaling changes were associated with decreased tissue abundance of interleukin-6 receptors and STAT3. In contrast to its effects on the janus kinase/STAT pathways, interleukin-6 activation of MAP kinases (extracellular signal-regulated kinase-1, extracellular signal-regulated kinase-2, and p38) was unaffected by endotoxin.

Conclusions: The pathway-specific inhibition of interleukin-6 signaling responses in the liver may be an important determinant of the pathophysiologic consequences of endotoxin exposure.