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Development and Application of a Fully Automated Chemiluminescence Enzyme Immunoassay for the Detection of Antibodies Against Porcine Circovirus 3 Cap.
Viruses
December 2024
State Key Laboratory of Swine and Poultry Breeding Industry, National Engineering Center for Swine Breeding Industry, College of Animal Science, South China Agricultural University, Guangzhou 510642, China.
Porcine circovirus 3 (PCV3) is a small non-enveloped circovirus associated with porcine dermatitis and nephropathy syndrome (PDNS). It has occurred worldwide and poses a serious threat to the pig industry. However, there is no commercially available vaccine.
View Article and Find Full Text PDFThe performance of a lateral flow SARS-CoV-2 antibody assay and semi-autonomous SARS-CoV-2 antisense and sense RNA fluorescence in situ hybridization assay in a prospective cohort pilot study within a Dutch military population.
PLoS One
December 2024
National Institute for Public Health and the Environment (RIVM), Centre for Infectious Diseases Research, Diagnostics and Laboratory Surveillance, Bilthoven, NLD.
At the beginning of the COVID-19 pandemic, diagnostic testing was not accessible for mildly ill or asymptomatic individuals. Military operational circumstances exclude the usage of reference laboratory tests. For that reason, at the beginning of the pandemic alternative test methods were needed in order to gain insight into the SARS-CoV-2 status of military personnel.
View Article and Find Full Text PDFLongitudinal determination of seroprevalence and immune response to SARS-CoV-2 in a population of food and retail workers through decentralized testing and transformation of ELISA datasets.
PLoS One
December 2024
Département de Chimie, Université de Montréal, Montréal, Canada.
Background: Since the onset of the global COVID-19 pandemic in early 2020, numerous studies have been conducted worldwide to understand our immune response to the virus and to vaccination. This study investigates the humoral response elicited by SARS-CoV-2 infection and by vaccination in the poorly studied population of food and retail workers. These occupations were classified as essential by the Public Health Agency of Canada, potentially placing this population at greater risk of infection.
View Article and Find Full Text PDFAutomated Dynamic Inlet Microfluidics (ADIM) system: cost-effective biaxial nanoliter droplet on demand generation platform and its application in agglutination assays.
Lab Chip
December 2024
School of Engineering, The University of British Columbia, Okanagan Campus, Kelowna, BC, Canada.
The paper demonstrates an adaptation of a Prusa Mini+ 3D printer through the integration of 3D printed modules, creating a system capable of producing varied droplets from multiple Eppendorf tubes. Building upon our previous model, this system enhances calibration methodology enabling any fused deposition modeling (FDM) printer to produce mono-disperse droplets (coefficient of variance (CV%) <2% for train of 100 droplets) with 6900 assays per hour rate. The cost of the developed system is 85% lower than that of existing droplet generation solutions on the market, and 30% more economical than the previous iteration of the system.
View Article and Find Full Text PDFComparison between rapid and laboratory serological tests in the context of the first responders during the SARS-CoV-2 outbreak: are the two tests interchangeable?
New Microbiol
November 2024
Department of Biomedicine and Prevention, University of Rome Tor Vergata, Rome, Italy.
Article Synopsis
- * A study was conducted using serological tests to evaluate the health of these first responders, comparing two types of tests: a lateral flow immunoassay (LFIA) and an automated laboratory immunoassay.
- * The study found a significant agreement (Cohen's kappa of 0.78) between the two test methods, and both tests exhibited high sensitivity at 97%.
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