We have examined the ability of selected hormones and growth factors to suppress the spontaneous onset on apoptotic DNA fragmentation in isolated vitellogenic rainbow trout ovarian follicles cultured in serum-free conditions. Primary culture of isolated follicles for 24 hr in serum-free conditions resulted in a 3-5-fold increase in the amount of fragmented DNA as compared to non-cultured controls, measured by radioactive 3'end-labeling. Culture in medium containing salmon gonadotropin (SG-G100; 1, 5 microg/ml) suppressed the spontaneous onset of DNA fragmentation in dose-dependent fashion. Culture with 1 ng/ml 17beta-estradiol, or 100 ng/ml epidermal growth factor also suppressed the spontaneous onset of apoptosis, whereas culture with higher concentrations of 17beta-estradiol (10 and 100 ng/ml), insulin-like growth factor I (IGF-I; 1, 10, and 100 ng/ml), or 8-bromo-cAMP (0.1, 1, and 5 mM) was ineffective in suppressing apoptosis. Apoptosis was confirmed as the mode of cell death through positive identification of nuclear morphological characteristics associated with apoptosis, and positive staining for fragmented DNA using in situ end-labeling (TUNEL); apoptotic cells identified in situ were almost exclusively localized to the thecal/epithelial region of the follicle. In summary, this study shows that vitellogenic ovarian follicles are susceptible to apoptosis and that both endocrine and locally-derived growth factors may play a role as cell survival factors by preventing apoptosis. The study also suggests that rainbow trout differ markedly from mammals both in terms of the cell types susceptible to apoptosis and the responsiveness to specific growth factors in terms of inhibiting apoptosis.
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http://dx.doi.org/10.1002/mrd.10108 | DOI Listing |
Abdom Radiol (NY)
January 2025
Jinshan Hospital of Fudan University, Shanghai, China.
Objective: This study aimed to investigate the diagnostic performance of Follicle numbers measured on ultrasound (US), conventional magnetic resonance imaging (2D MRI), and three-dimensional (3D) MRI in patients with polycystic ovary syndrome (PCOS) and to compare the diagnostic efficacy of these imaging modalities.
Method: In this prospective study, 58 PCOS patients and 60 healthy women underwent US, conventional 2D MRI, and 3D MRI. Clinical laboratory tests and ovarian volume were compared between PCOS and control groups.
BMJ
January 2025
State Key Laboratory of Reproductive Medicine and Offspring Health, Centre for Reproductive Medicine, Institute of Women, Children and Reproductive Health, Shandong University, Jinan, China
Objective: To test the hypothesis that a freeze-all strategy would increase the chance of live birth compared with fresh embryo transfer in women with low prognosis for in vitro fertilisation (IVF) treatment.
Design: Pragmatic, multicentre, randomised controlled trial.
Setting: Nine academic fertility centres in China.
Ginekol Pol
January 2025
Department of Clinical Dietetics, Faculty of Health Sciences, Medical University of Warsaw, Poland, Poland.
Anti-Müllerian hormone (AMH), also known as Müller duct inhibitory factor and primarily known for its role in sexual differentiation. In female fetuses, AMH production by granulosa cells begins around the 36th week of gestation and continues in women until menopause. It is becoming more significant in the endocrine and gynecological diagnosis of adult women.
View Article and Find Full Text PDFJ Ovarian Res
January 2025
Department of Reproductive Medicine Center, Second Affiliated Hospital of Naval Medical University, Shanghai, 200003, China.
Background: Polycystic ovary syndrome (PCOS) is an endocrine disease associated with reproductive and metabolic abnormalities. The aim of this study was to elucidate the effects of Schisandra rubriflora (S. rubriflora) on PCOS and its related mechanisms using network pharmacology, molecular docking and in vitro experiments.
View Article and Find Full Text PDFSci Rep
January 2025
Department of Obstetrics and Gynecology, The Second Hospital of Jilin University, No. 4110 Yatai Street, Nanguan District, Changchun, Jilin, 130000, China.
Polycystic ovary syndrome (PCOS) is a complex gynecological endocrinological condition that significantly impacts women's fertility during their reproductive lifespan. The causes of PCOS are multifaceted, and its pathogenesis is not yet clear. This study established a rat model of PCOS and, in conjunction with clinical samples and database data, analysed the role of claudin 11 (CLDN11) in follicular granulosa cells (GCs) in regulating the proliferation of GCs.
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