There are about 50 copies of each chromosome in the somatic macronucleus of the ciliated protozoan TETRAHYMENA: Approximately 0.8% of the adenine residues in the macronuclear DNA of Tetrahymena are methylated to N6-methyladenine. The degree of methylation varies between sites from a very low percentage to >90%. In this study a correlation was found between nucleosome positioning and DNA methylation. Eight GATC sites with different levels of methylation were examined. There was a direct correlation between the degree of methylation and proximity to linker DNA at these sites. Although methylation occurs preferentially in linker DNA, the patterns and extent of methylation in a histone H1 knockout strain were virtually indistinguishable from those in wild-type cells.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC101364 | PMC |
| http://dx.doi.org/10.1093/nar/30.6.1364 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
The "Ins and Outs and What-Abouts" of H2A.Z: A Tribute to C. David Allis.
J Biol Chem
January 2025
Institute for Genetics, Justus-Liebig-University Giessen, Heinrich-Buff-Ring 58-62, 35390 Giessen, Germany. Electronic address:
In 2023, the brilliant chromatin biologist C. David Allis passed away leaving a large void in the scientific community and broken hearts in his family and friends. With this review, we want to tribute Dave's enduring inspiration by focusing on the histone variant H2A.
View Article and Find Full Text PDFAssessing the impact of three fish drugs on tetrahymenosis in Fish using environmental DNA techniques.
Vet Parasitol
November 2024
Laboratory of Protozoology, Harbin Normal University, Harbin 150025, China. Electronic address:
Article Synopsis
- Tetrahymenosis has caused significant economic losses in the ornamental fish farming industry in Harbin, prompting research into treatments for affected fish.
- Previous studies focused on disease outbreaks, but this experiment evaluates the effectiveness of three specific fish drugs (copper, Nitrofural, Methylene Blue) in treating tetrahymenosis in goldfish.
- Using advanced genetic sequencing and analysis, the study found that Nitrofural was the most effective treatment, followed by Methylene Blue, whereas copper sulfate was the least effective, providing insights into treatment options and the disease's impact on goldfish health.
Analyzing the Interaction between and Bacteria under Different Physicochemical Conditions When Infecting Guppy Using the eDNA Method.
Animals (Basel)
July 2024
Laboratory of Protozoology, Harbin Normal University, Harbin 150025, China.
Article Synopsis
- In the study of guppy fish in aquaculture, researchers looked at how bacteria and tiny parasites interact and how this affects fish health.
- Different water conditions, like temperature and pH, were tested to see how they impact these interactions.
- The findings showed that certain bacteria were linked to fish dying, and cooler temperatures with higher pH levels helped reduce diseases in the fish.
Semiconservative transmission of DNA -adenine methylation in a unicellular eukaryote.
Genome Res
June 2024
Department of Biochemistry and Molecular Medicine, Keck School of Medicine, University of Southern California, Los Angeles, California 90033, USA;
Although DNA -adenine methylation (6mA) is best known in prokaryotes, its presence in eukaryotes has recently generated great interest. Biochemical and genetic evidence supports that AMT1, an MT-A70 family methyltransferase (MTase), is crucial for 6mA deposition in unicellular eukaryotes. Nonetheless, the 6mA transmission mechanism remains to be elucidated.
View Article and Find Full Text PDFIdentification of a Tetrahymena species infecting guppies, pathology, and expression of beta-tubulin during infection.
Parasitol Res
January 2024
Laboratory of Protozoology, Harbin Normal University, Harbin, 150025, China.
Tetrahymenosis is caused by the ciliated protozoan Tetrahymena and is responsible for serious economic losses to the aquaculture industry worldwide. However, information regarding the molecular mechanism leading to tetrahymenosis is limited. In previous transcriptome sequencing work, it was found that one of the two β-tubulin genes in T.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!