The adjuvant properties of subalin, a recombinant probiotic prepared from live bacteria Bacillus subtilis producing human alpha 2-interferon were studied in the scheme of its use with vaccines against parvovirus enteritis and distemper. Subalin was shown to be capable of preventing immunosuppression caused by the injection of vaccines, accelerating the formation of the antigen-specific clone of memory cells and enhancing antigen-specific immune response. The mechanisms of the adjuvant effect of subalin were considered; this effect was shown to be due to the action of interferon excreted by bacteria of B. subtilis into the lumen of the intestine. The advantages of this method of interferon supply and the prospects of using subalin preparation as adjuvant are discussed.
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Efficiency of a subalin probiotic drug created on the basis of live microbic cultures was investigated, at acute alcoholic intoxication developed in experimental animals. It was shown that after one time administration of this drug to animals there was no considerable influence on activity of the main enzymes of ethanol metabolism--alcohol- and aldehyde dehydrogenase both in animals with an alcoholic intoxication and without. However subalin induced considerable changes in the quantitative maintenance of acetaldehyde in blood of animals with alcoholic intoxication, which concentration decreased almost in 20 times.
View Article and Find Full Text PDF[Biological effects of interferon, produced by recombinant bacteria of the probiotic preparation subalin].
Zh Mikrobiol Epidemiol Immunobiol
December 2003
State Research Center Vector, Koltsovo, Novosibirsk Region.
The present review deals with the analysis of biological and functional activities of recombinant bacteria Bacillus subtilis IF-alpha 2335 are producing a human interferon. The interferon-producing bacteria are constructed on a basis commercial probiotic strain B.subtilis 2335, carrying a recombinant plasmid pMBM 105 with the gene of human alpha-2 interferon.
View Article and Find Full Text PDF[Adjuvant properties of subalin, a recombinant interferon-producing probiotic].
Zh Mikrobiol Epidemiol Immunobiol
May 2002
State Research Center Vecto, Koltsovo, Novosibirsk Region, Russia.
The adjuvant properties of subalin, a recombinant probiotic prepared from live bacteria Bacillus subtilis producing human alpha 2-interferon were studied in the scheme of its use with vaccines against parvovirus enteritis and distemper. Subalin was shown to be capable of preventing immunosuppression caused by the injection of vaccines, accelerating the formation of the antigen-specific clone of memory cells and enhancing antigen-specific immune response. The mechanisms of the adjuvant effect of subalin were considered; this effect was shown to be due to the action of interferon excreted by bacteria of B.
View Article and Find Full Text PDFSuch biological factors as bile, gastric juice, blood serum, amino acids and pH of the medium have been studied for their effect on the growth intensity and antagonistic activity of bacilli being the basis of biosporin and subalin. It has been established that pH of the medium within 7.0-9.
View Article and Find Full Text PDFApproaches to designing a new probiotic class based on recombinant strains of bacteria that produce the predetermined therapeutic proteins are dealt with. The prospects of the approach are shown via studies of the biological properties of Bacillus subtilis 2335 strain transformed by the plasmid encoding the synthesis of human interferon alpha-2. The recombinant strain was demonstrated to preserve the high antagonistic activity of the parent culture (the bases of the probiotic biosporine) and to acquire marked antiviral properties due to interferon synthesis.
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