AI Article Synopsis
- Molecular analysis of mitochondrial DNA (mtDNA) can help diagnose mitochondrial diseases, and this study evaluated whether the Agilent 2100 bioanalyzer could replace traditional methods like RFLP analysis on agarose gels.
- Three family members with MELAS syndrome and four with MERRF syndrome underwent DNA testing, comparing results from the bioanalyzer and conventional methods.
- Findings indicated that the bioanalyzer produced results with lower error rates and better reproducibility, suggesting it could effectively replace the current methods for detecting mtDNA mutations.
Article Abstract
Background: Molecular analysis of mitochondrial DNA (mtDNA) has provided a final diagnosis for many of the mitochondrial diseases. We evaluated the Agilent 2100 bioanalyzer (Agilent Technologies, Palo Alto, CA) to determine whether the system could replace the conventional restriction fragment length polymorphism (RFLP) analysis by the agarose gel electrophoresis for the detection of the mtDNA mutation.
Methods: Three members of a family with MELAS syndrome and four members of a family with MERRF syndrome were recruited for this study. After PCR and restriction enzyme digestion, DNA fragments were separated on the Agilent 2100 bioanalyzer in conjunction with the DNA 500 and DNA 1000 Labchip kits and by electrophoresis on precast 3% agarose gels.
Results: The data generated by the DNA 500 and DNA 1000 assays using the Agilent 2100 bioanalyzer showed a lower percentage error and a better reproducibility as compared to those obtained by the conventional method.
Conclusion: Based on the performance of the bioanalyzer, we suggest that this novel Labchip is adequate to replace the current RFLP analysis by the agarose gel electrophoresis for mtDNA mutation detection.
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| http://dx.doi.org/10.1016/s0009-8981(01)00809-9 | DOI Listing |
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