AI Article Synopsis
- Prodigiosin (PG), a red pigment from Serratia marcescens, has cytotoxic and immunosuppressive properties, particularly inducing apoptosis in cancer cells like Jurkat-T.
- Time course experiments revealed that the phosphorylation of p38-MAPK starts at 15 minutes and continues for 3 hours, while JNK remains unphosphorylated despite being present.
- The study suggests PG specifically activates p38-MAPK, leading to increased expression of c-jun and c-fos oncoproteins, which are involved in cell growth and survival.
Article Abstract
Prodigiosin (PG) is a red pigment produced by Serratia marcescens, with cytotoxic and immunosuppressive activity. It induces apoptosis in several cancer cell lines, including Jurkat-T cells. Here we examine the role of two stress-stimulated kinase cascades in this induction. Time course experiments using polyclonal antibodies showed that p38-MAPK phosphorylation began at 15 min and lasted for 3 h, whereas JNK was not phosphorylated, although both proteins were present. SB203580, a selective inhibitor of p38-MAPK, blocked its phosphorylation in PG-treated cells. Taken together, these data suggest that the PG induces phosphorylation of p38-MAPK but not of SAPK/JNK and that it increases the expression of both c-jun and c-fos oncoproteins.
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| http://dx.doi.org/10.1016/s0378-4274(01)00477-5 | DOI Listing |
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