Wheat (Triticum aestivum) plants were stably transformed with the coat protein (CP) gene of wheat streak mosaic virus (WSMV) by the biolistic method. Eleven independently transformed plant lines were obtained and five were analyzed for gene expression and resistance to WSMV. One line showed high resistance to inoculations of two WSMV strains. This line had milder symptoms and lower virus titer than control plants after inoculation. After infection, new growth did not show symptoms. The observed resistance was similar to the 'recovery' type resistance described previously using WSMV NIb transgene and in other systems. This line looked morphologically normal but had an unusually high transgene copy number (approximately 90 copies per 2C homozygous genome). Northern hybridization analysis indicated a high level of degraded CP mRNA expression. However, no coat protein expression was detected.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1023/a:1013944011049 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
The transcription factor SlLBD40 regulates seed germination by inhibiting cell wall remodeling enzymes during endosperm weakening.
Plant Physiol
January 2025
College of Horticulture, China Agricultural University, Beijing 100193, China.
Uniform seed germination is crucial for consistent seedling emergence and efficient seedling production. In this study, we identified a seed-expressed protein in tomato (Solanum lycopersicum), lateral organ boundaries domain 40 (SlLBD40), that regulates germination speed. CRISPR/Cas9-generated SlLBD40 knockout mutants exhibited faster germination due to enhanced seed imbibition, independent of the seed coat.
View Article and Find Full Text PDFFirst complete genome sequence of tulip mild mottle mosaic virus (Ophiovirus tulipae).
Arch Virol
January 2025
School of Agriculture, Utsunomiya University, 350 Mine-machi, Utsunomiya, Tochigi, 321-8505, Japan.
Tulip mild mottle mosaic disease, caused by tulip mild mottle mosaic virus (TMMMV, species Ophiovirus tulipae), was first reported in Japan in 1979. TMMMV has a negative-sense ssRNA genome and is closely related to ophioviruses such as Mirafiori lettuce big vein virus (MLBVV, Ophiovirus mirafioriense). However, its complete nucleotide sequence has not yet been reported.
View Article and Find Full Text PDFSequence analysis and genome organization of a new marafivirus from Leptochloa chinensis.
Arch Virol
January 2025
State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fujian Agriculture and Forestry University, Fuzhou, 350002, Fujian, China.
High-throughput sequencing was used to identify and characterize a novel marafivirus from the weed Leptochloa chinensis, which was tentatively named "Leptochloa chinensis marafivirus" (LcMV). The complete genome of the virus consists of 6,178 base pairs, and its nucleotide sequence is 73.82% identical to that of Sorghum almum marafivirus, which is a member of the genus Marafivirus within the family Tymoviridae.
View Article and Find Full Text PDFFabrication of Versatile Antifouling Coatings Inspired by Melanogenesis Using a Tyrosine-Conjugated Carboxybetaine Derivative.
Langmuir
January 2025
Department of Chemistry and Chemistry Institute for Functional Materials, Pusan National University, Busan 46241, Republic of Korea.
In this study, we developed zwitterionic surface coatings of carboxybetaine by mimicking natural melanogenesis. We synthesized an unnatural tyrosine-conjugated carboxybetaine (Tyr-CB) that undergoes melanin-like oxidation upon treatment with tyrosinase under various aqueous conditions. The thickness of the resulting poly(Tyr-CB) film was tuned by adjusting the pH during the coating process.
View Article and Find Full Text PDFTransient interactions between the fuzzy coat and the cross-β core of brain-derived Aβ42 filaments.
Sci Adv
January 2025
Centre for Misfolding Diseases, Department of Chemistry, University of Cambridge, Cambridge CB2 1EW, UK.
Several human disorders, including Alzheimer's disease (AD), are characterized by the aberrant formation of amyloid fibrils. In many cases, the amyloid core is flanked by disordered regions, known as fuzzy coat. The structural properties of fuzzy coats, and their interactions with their environments, however, have not been fully described to date.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!