AI Article Synopsis
- Researchers isolated Helicobacter pylori from wastewater using methods like immunomagnetic separation, cell culture, and PCR verification of 16S rRNA sequences.
- Eleven genotyped H. pylori isolates were categorized into four vacA classes, with the majority being of the vacA s1a/m1 type, linked to severe diseases like gastric cancer.
- The findings highlight that H. pylori can survive in water systems, suggesting untreated water could be a source of H. pylori transmission.
Article Abstract
For this study, we isolated Helicobacter pylori from wastewater by a series of steps beginning with immunomagnetic separation and cell culture. After Gram staining and three standard microbial tests, the 16S rRNA sequences of a total of 23 out of 37 putative H. pylori isolates were verified by PCR. Eleven H. pylori isolates were genotyped and fell into four vacA classes: those with the vacA allelic variants s1a and m1, s1b and m1, s2 and m2, or s2 and m1. Most H. pylori isolates were of the vacA s1a/m1 type, which has been shown to be associated with advanced diseases based on genotyping of H. pylori from gastric cancer patients. These results demonstrated that H. pylori survives in water and may be a potential source of H. pylori transmission, especially where water is not adequately treated.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC123781 | PMC |
| http://dx.doi.org/10.1128/AEM.68.3.1436-1439.2002 | DOI Listing |
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