OBJECTIVE: To assess restriction endonuclease analysis (REA) of chromosomal DNA using SalI enzyme, low-concentration (0.4%) agarose gels and digitalized data management of the REA patterns obtained for the typing of clinical Pseudomonas aeruginosa isolates. METHODS: A group of 67 clinical unrelated isolates from 10 Spanish hospitals was used to study the discriminatory power, reproducibility and typeability of REA typing. RESULTS: A SalI REA pattern consisted of a variety (1--10) of restriction bands in the range between 12.2 and 48.5 kb and an unresolvable smear of low-molecular-weight bands. Forty different SalI REA patterns with an index of discrimination of 0.979 were obtained. Low typeability (91.04%) was the major limitation of REA typing. Analysis of blinded subcultures of eight Pseudomonas aeruginosa strains showed the reproducibility of REA typing to be 87.5%. Combined phenotypic typing (O-serotyping and phage typing) performed on the same group of strains showed comparable discrimination but much lower reproducibility. Isolates selected from five clusters of nosocomial infections in hospitals in the UK were typed by REA typing, and the results show high agreement when compared with conventional phenotypic typing methods in distinguishing between strains. CONCLUSIONS: These data underline the usefulness of REA typing enhanced with digitalized data management for the epidemiologic subtyping of clinical Pseudomonas aeruginosa isolates.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1111/j.1469-0691.1997.tb00601.x | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Identification of Aichivirus in a Pet Rat () in Italy.
Animals (Basel)
June 2024
Istituto Zooprofilattico Sperimentale del Mezzogiorno, 80055 Portici, Italy.
Article Synopsis
- The study examined 91 exotic companion mammals from pet shops in southern Italy for the presence of eight potential zoonotic viruses using advanced PCR techniques.
- Only one rat tested positive for aichivirus, which showed a close genetic relationship to a strain identified in China, indicating possible introduction from Asia.
- Continuous monitoring of these animals is essential to prevent the spread of pathogens to humans and domestic animals, emphasizing the need for further research on zoonotic viruses.
Development and evaluation of genomics informed real-time PCR assays for the detection and strain typing of Mycobacterium avium subsp. paratuberculosis.
J Appl Microbiol
May 2024
Agriculture Victoria, AgriBio, La Trobe University, Bundoora, Victoria 3083, Australia.
Aims: This study aimed to identify specific genomic targets for the detection and strain typing of Map and analyse their sensitivity and specificity, and detect Map directly from faeces.
Methods And Results: A comparative genomics approach was used to identify specific genomic targets for the detection and strain typing of Map. A Map specific qPCR using the primer pair 7132 that targets a DNA segregation ATPase protein was able to detect all strains of Map and is more sensitive than the current Johne's disease PCR assays with a sensitivity of 0.
The serological and genetic diversity of the Icterohaemorrhagiae serogroup circulating in the UK.
J Vet Res
December 2023
Veterinary Sciences Division, Agri-Food and Biosciences Institute, Stormont, Belfast BT4 3SD, UK.
Introduction: Strains of belonging to two very closely related serovars, Icterohaemorrhagiae and Copenhageni, have been associated with disease in mammalian species and are the most frequently reported agents of human leptospirosis. They are considered the most pathogenic serovars and represent more than half of the leptospires encountered in severe human infections.
Material And Methods: Nineteen such isolates from the United Kingdom - human, domestic and wildlife species - were typed using three monoclonal antibodies (F12 C3, F70 C14 and F70 C24) in an attempt to elucidate their epidemiology.
Molecular characterization of human adenovirus causing infantile acute gastroenteritis in Venezuela before and after rotavirus vaccine implementation.
Diagn Microbiol Infect Dis
November 2023
Laboratorio de Biología de Virus, Centro de Microbiología y Biología Celular (CMBC), Instituto Venezolano de Investigaciones Científicas (IVIC), Caracas, Venezuela. Electronic address:
Article Synopsis
- Human adenoviruses (HAdV) of species F are linked to stomach illnesses in kids (AGE) in Venezuela.
- Researchers tested 630 poop samples from kids with AGE and found HAdV in 123 cases, mostly in girls younger than 2.
- The study shows that HAdV-F41 is very common and highlights the need to monitor other viruses, especially since kids who got the rotavirus vaccine had higher rates of HAdV infection.
Effect of the COVID-19 Pandemic on Rates and Epidemiology of Infection in One VA Hospital.
Antibiotics (Basel)
July 2023
Edward Hines Jr. VA Hospital, 5000 S. 5th Ave., Hines, IL 60141, USA.
The COVID-19 pandemic was associated with increases in some healthcare-associated infections. We investigated the impact of the pandemic on the rates and molecular epidemiology of infection (CDI) within one VA hospital. We anticipated that the potential widespread use of antibiotics for pneumonia during the pandemic might increase CDI rates given that antibiotics are a major risk for CDI.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!