AI Article Synopsis
- The Norwalk virus capsid protein was successfully expressed using Venezuelan equine encephalitis virus replicon particles, resulting in the production of numerous NV-like particles mostly associated with cells.
- The recombinant NV-like particles generated were similar in appearance to those made from baculovirus systems.
- Mutations in the N-terminal and P1 regions of the NV capsid protein hindered its ability to self-assemble in mammalian cells.
Article Abstract
The Norwalk virus (NV) capsid protein was expressed using Venezuelan equine encephalitis virus replicon particles (VRP-NV1). VRP-NV1 infection resulted in large numbers of recombinant NV-like particles that were primarily cell associated and were indistinguishable from NV particles produced from baculoviruses. Mutations located in the N-terminal and P1 domains of the NV capsid protein ablated capsid self-assembly in mammalian cells.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC135954 | PMC |
| http://dx.doi.org/10.1128/jvi.76.6.3023-3030.2002 | DOI Listing |
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