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Characterization of a monoclonal antibody with specificity for holo-transcobalamin.
Nutr Metab (Lond)
January 2006
Axis-Shield AS, POB 206 Økern, N-0510 Oslo, Norway.
Background: Holotranscobalamin, cobalamin-saturated transcobalamin, is the minor fraction of circulating cobalamin (vitamin B12), which is available for cellular uptake and hence is physiologically relevant. Currently, no method allows simple, direct quantification of holotranscobalamin. We now report on the identification and characterization of a monoclonal antibody with a unique specificity for holotranscobalamin.
View Article and Find Full Text PDFQuantification of holo-transcobalamin, a marker of vitamin B12 deficiency.
Clin Chem
March 2002
Department of Clinical Biochemistry, Aarhus University Hospital, DK-8000 Aarhus C, Denmark.
Direct assay for cobalamin bound to transcobalamin (holo-transcobalamin) in serum.
Clin Chem
March 2002
Axis-Shield ASA, Ulvenveien 87, PO Box 206 Økern, N-0510 Oslo, Norway.
Background: Only cobalamin carried by transcobalamin (holo-transcobalamin) is available for cellular uptake and hence is physiologically relevant. However, no reliable or accurate methods for quantifying holo-transcobalamin are available. We report a novel holo-transcobalamin assay based on solid-phase capture of transcobalamin.
View Article and Find Full Text PDFApplication of heparin-conjugated Sepharose for the measurement of cobalamin-saturated and unsaturated transcobalamin II.
Clin Chim Acta
March 1988
Institute of Hematology, Erasmus University, Rotterdam, The Netherlands.
The cobalamin-binding plasma protein transcobalamin II has a high affinity for the anticoagulant heparin. This phenomenon has been exploited in a new method for the quantification of cobalamin-saturated (holo-) and unsaturated (apo-) transcobalamin II in human plasma. Transcobalamin II is adsorbed from human plasma to heparin-conjugated Sepharose under suitable conditions and either cobalamin from adsorbed holo-transcobalamin II is measured by a radioisotope dilution assay or apo-transcobalamin II is determined by measuring the adsorbed unsaturated cobalamin-binding capacity with radioactive cobalamin.
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