AI Article Synopsis
Article Abstract
The amphibian oocyte cell model is widely used for heterologous expression of ionic channels and receptors. Little is known, however, about the physiology of oocyte cell models other than Xenopus laevis. In this study, the two-electrode voltage clamp technique was used to assess the most common electrical patterns of oocytes of the South American toad Bufo arenarum. Basal membrane resistance, resting potential, and ionic currents were determined in this cell model. The oocyte transmembrane resistance was 0.35 M(Omega), and the resting potential in normal saline was about -33 mV with a range between -20 mV and -50 mV. This is, to our knowledge, the first attempt to begin an understanding of the ion transport mechanisms of Bufo arenarum oocytes. This cell model may provide a viable alternative to the expression of ion channels, in particular those endogenously observed in Xenopus laevis oocytes.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1002/jez.10062 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Toxicological assessment of the effects of CuCl and CuO nanoparticles on early developmental stages of the South American toad, Rhinella arenarum by standardized bioassays.
Environ Sci Pollut Res Int
December 2024
IIIA-UNSAM-CONICET, Instituto de Investigación E Ingeniería Ambiental, Escuela de Hábitat y Sostenibilidad (EHyS), Universidad Nacional de San Martín (UNSAM), Av. 25 de Mayo 1021 (CP. 1650), Buenos Aires, Argentina.
The release in aquatic environments of emergent contaminants such as copper oxide nanoparticles (CuO-NPs) has generated concerns on their short- and long-term toxicity and the potential risk for more vulnerable animal groups, such as amphibians. In this sense, the aim of this work was to evaluate the toxicity of CuO-NPs in comparison with its respective salt (CuCl) in embryos and larvae of a native amphibian, Rhinella arenarum, by acute (96 h) and chronic (504 h) standardized bioassays. Lethality and sublethal effects such as developmental, morphological, and ethological alterations were assessed in a wide range of concentrations (0.
View Article and Find Full Text PDFRunway extinction in terrestrial toads (Rhinella arenarum): Instrumental or Pavlovian?
Behav Processes
August 2024
Instituto de Biología y Medicina Experimental, Consejo Nacional de Investigaciones Científicas y Técnicas (IBYME-CONICET), Vuelta de Obligado 2490, 1428 Buenos Aires, Argentina; Universidad de Buenos Aires, Facultad de Psicología, Instituto de Investigaciones,Lavalle 2353,1052 Buenos Aires, Argentina.
Instrumental appetitive extinction involves the reduction of a previously reinforced response when its occurrence is no longer rewarded. Two experiments with terrestrial toads (Rhinella arenarum) tested whether the occurrence of a nonreinforced response is necessary for response extinction by varying the time of exposure to nonrewarded goal-box stimuli across groups. In Experiment 1, toads that received the same acquisition training (15 sessions, 1 session/day, 300 s of access to water in the goal box) were randomly assigned to two groups.
View Article and Find Full Text PDFA multi-approach analysis of the toxicity of a commercial formulation of monensin on Rhinella arenarum embryos and larvae.
Environ Toxicol Pharmacol
June 2024
IIIA-UNSAM-CONICET, Instituto de Investigación e Ingeniería Ambiental, Escuela de Hábitat y Sostenibilidad, Campus Miguelete, 25 de mayo y Francia, San Martín, Provincia de Buenos Aires 1650, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina. Electronic address:
Monensin, an antibacterial commonly used in animal fattening, can enter aquatic ecosystems and harm non-target organisms. Since there are no previous studies about the effects of monensin on amphibians, the aim of the present study was to evaluate the lethal and sublethal toxicity of a commercial formulation of monensin (CFM) through standardized bioassays with embryos and larvae of the amphibian Rhinella arenarum. Oxidative stress (catalase and glutathione S-transferase activities, and reduced glutathione and lipid peroxidation levels), cholinesterasic effect (acetylcholinesterase and butyrylcholinesterase activities) and mutagenicity (micronuclei frequency) biomarkers were evaluated.
View Article and Find Full Text PDFImpacts of horticultural environments on Rhinella arenarum (Anura, Bufonidae) populations: exploring genocytotoxic damage and demographic life history traits.
Environ Sci Pollut Res Int
March 2024
Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina.
Horticulture poses a significant ecological risk, as agrochemicals are applied more frequently and in larger quantities per unit of production compared to extensive crop fields. The native amphibian Rhinella arenarum serves as a reliable bioindicator of environmental health. This study aimed to assess genocytotoxic damage and demographic life history traits of R.
View Article and Find Full Text PDFToxicity assessment of a tebuconazole-based fungicide on the embryo-larval development of the common south American toad Rhinella arenarum.
Environ Toxicol
April 2024
IIIA-UNSAM-CONICET, Instituto de Investigación e Ingeniería Ambiental, Escuela de Hábitat y Sostenibilidad, Campus Miguelete, San Martín, Provincia de Buenos Aires, Argentina.
Agrochemicals cause diverse effects on aquatic communities, and amphibian species are particularly threatened due the high susceptibility to contamination. Present study evaluates the toxicity of a widely used fungicide tebuconazole (Trigal®) by the assessment of mortality and developmental alterations at acute, subchronic, and chronic exposure during the embryo-larval development of the South American toad Rhinella arenarum. Also, the sensitivity of the different embryonic stages was evaluated with 24-h pulse exposure treatments.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!