AI Article Synopsis
- The heat shock protein DnaK is critical for the ability of Brucella suis to replicate within macrophages.
- Replacing the native dnaK promoter with a constant promoter allowed DnaK production regardless of temperature, enabling growth at 37°C but with limited success at higher temperatures.
- This constitutive mutant struggled in conditions mimicking stress and couldn't replicate or survive in macrophages or in a mouse infection model, supporting the idea that DnaK's stress-induced expression is vital for B. suis survival and replication.
Article Abstract
The heat shock protein DnaK is essential for intramacrophagic replication of Brucella suis. The replacement of the stress-inducible, native dnaK promoter of B. suis by the promoter of the constitutively expressed bla gene resulted in temperature-independent synthesis of DnaK. In contrast to a dnaK null mutant, this strain grew at 37 degrees C, with a thermal cutoff at 39 degrees C. However, the constitutive dnaK mutant, which showed high sensitivity to H(2)O(2)-mediated stress, failed to multiply in murine macrophage-like cells and was rapidly eliminated in a mouse model of infection, adding strong arguments to our hypothesis that stress-mediated and heat shock promoter-dependent induction of dnaK is a crucial event in the intracellular replication of B. suis.
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Source |
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC127800 | PMC |
| http://dx.doi.org/10.1128/IAI.70.3.1631-1634.2002 | DOI Listing |
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