OBJECTIVE: To examine the effect of recombinant human IL-10 (rhIL-10) on MAC infection of human macrophages and C57BL/6 mice. METHODS: We compared rhIL-10 with the effects of the immunosuppressive drugs prednisolone and cyclosporin A, both in vitro and in vivo. RESULTS: There was no effect of rhIL-10 on the multiplication of MAC in human macrophages after 1 week of infection. In C57BL/6 mice, rhIL-10 at 2.5 or 25 m g/mouse had no additional multiplicatory effect after 3 weeks of infection, while the spleens of mice treated with prednisolone had 600% higher bacteria than controls or rhIL-10-treated mice (p0.01). CONCLUSIONS: These data suggest that rhIL-10 does not further decrease the resistance of human macrophages and C57BL/6 mice to MAC infection, whereas prednisolone leads to increased multiplication of MAC in the spleens of infected C57BL/6 mice. These results may be of interest in the context of the therapeutic use of rhIL-10 in some autoimmune disorders.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1111/j.1469-0691.1999.tb00435.x | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Acute pancreatitis (AP) is a life-threatening condition, with a higher mortality rate in men than women and in which estrogens might play a protective role. This study aimed to investigate sex-dependent differences in a mouse model of caerulein-induced AP. Thirty-six C57BL/6J mice (19 females and 17 males) were treated intraperitoneally with phosphate-buffered saline or caerulein, and sacrificed 12 hours, 2 days, or 7 days after the last injection.
View Article and Find Full Text PDFTerminal complement complex deposition on chondrocytes promotes premature senescence in age- and trauma-related osteoarthritis.
Front Immunol
January 2025
Division for Biochemistry of Joint and Connective Tissue Diseases, Department of Orthopedics, Ulm University Medical Center, Ulm, Germany.
Background: The complement system is locally activated after joint injuries and leads to the deposition of the terminal complement complex (TCC). Sublytic TCC deposition is associated with phenotypical alterations of human articular chondrocytes (hAC) and enhanced release of inflammatory cytokines. Chronic inflammation is a known driver of chondrosenescence in osteoarthritis (OA).
View Article and Find Full Text PDFCannabidiol Ameliorates Doxorubicin-Induced Myocardial Injury via Activating Hippo Pathway.
Drug Des Devel Ther
January 2025
School of Basic Medicine, Jiamusi University, Jiamusi, Heilongjiang, 154000, People's Republic of China.
Background: Doxorubicin (DOX) is a chemotherapeutic agent widely used for cancer treatment and has non-negligible cardiotoxicity. Some previous studies have reported that cannabidiol (CBD) has cardioprotective effects. In this study, we evaluated the protective effects of CBD against DOX-induced cardiomyocyte injury, and explored the downstream molecular mechanism.
View Article and Find Full Text PDFPinocembrin alleviates renal ischemia-reperfusion injury/unilateral ureteral obstruction (UUO)-generated renal fibrosis by targeting the CYP1B1/ROS/MAPK axis.
FEBS J
January 2025
Department of Urology, Renmin Hospital of Wuhan University, China.
In our research, we constructed models of renal ischemia-reperfusion (I/R)-exposed acute kidney injury (AKI) and unilateral ureteral obstruction (UUO)-stimulated renal fibrosis (RF) in C57BL/6 mice and HK-2 cells. We firstly authenticated that oral pinocembrin (PIN) administration obviously mitigated tissue damage and renal dysfunction induced by I/R injury, and PIN attenuated UUO-caused RF, as confirmed by the reduced expression of fibrotic markers as well as hematoxylin-eosin (H&E), Sirius red, immunohistochemistry, and Masson staining. Meanwhile, the beneficial role of PIN was again demonstrated in HK-2 cells with hypoxia-reoxygenation (H/R) or transforming growth factor beta-1 (TGF-β1) treatment.
View Article and Find Full Text PDFHilbert transform analysis of the mouse scotopic electroretinogram reveals two distinct bursts of oscillatory potentials with progressively dimmer flashes.
Doc Ophthalmol
January 2025
Department of Ophthalmology and Visual Sciences, Research Institute of the McGill University Health Centre/Montreal Children's Hospital, 1001 Décarie Boulevard, Glen Site, Block E, Office #EM03238, Montréal, QC, H4A 3J1, Canada.
Purpose: Study the scotopic oscillatory potentials (OPs) in mice over a wide range of flash luminance levels using the Hilbert transform (HT) to extract new features of the high frequency components of the electroretinogram (ERG).
Methods: Scotopic ERGs [Intensity: - 6.3 to 0.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!