AI Article Synopsis
- The text discusses a new method for tagging cloned genes using a mini-Tn10 transposon delivery vector for efficient epitope tagging.
- This system was successfully tested on a lambdaFIX genomic clone of the human nucleolin gene in HeLa cells.
- Results showed that the transfected cells produced both the correctly spliced tagged RNA and the properly localized tagged protein.
Article Abstract
Here, we describe an efficient system for epitope tagging cloned genes by CD tagging using a mini-Tn10 transposon delivery vector. The system was tested against a lambdaFIX genomic clone of the human nucleolin gene. Transfection of HeLa cells with the tagged gene led to the expression of both the appropriately spliced tagged transcript and the appropriately localized tagged protein.
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| http://dx.doi.org/10.2144/02322rr04 | DOI Listing |
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