Tumor necrosis factor-alpha gene polymorphisms in relation to periodontitis.

Background: Genetic polymorphisms for cytokines have been proposed as potential genetic markers for destructive periodontal disease. The present aim was to investigate 4 bi-allelic polymorphisms in the TNF-alpha gene in relation to susceptibility for and severity of periodontitis. The polymorphisms were all transitions from G to A, 3 in the promoter positions: -376, -308, -238; and one in the first intron at position +489.

Methods: 90 periodontitis patients and 264 reference control subjects were genotyped and frequencies of genotypes and alleles were analyzed. Also genotype frequencies among severe and moderate periodontitis patients were explored, and the % of teeth with 50% bone loss and the % of teeth without any bone loss for patients with the A allele (A+ genotype) and patients without (A- genotype) were compared.

Results: The distributions of genotypes and frequencies of A allele carriage rates for the 4 TNF-alpha polymorphisms were not different between patients and reference controls; Hardy-Weinberg equilibrium criteria were fulfilled. Also the distribution of A+ and A- genotypes, alone or in combinations, were not different for severe periodontitis compared to moderate periodontitis patients. None of the A+ genotypes showed a significant different bone loss pattern compared to A- genotype patients. Smoking status of the patients did not influence the results.

Conclusions: Genetic polymorphisms in the TNF-alpha gene at positions -376, -308, -238 and +489 could not be identified as susceptibility or severity factors in periodontitis, irrespective of the smoking status of the patients.