Here we describe the molecular cloning of human cyclin B3, its localization, and its structure. It is localized in the subcentromeric region of the X chromosome, still not completely sequenced by the Human Genome Project. This cyclin B3 is unusually large for a mitotic cyclin. Its mRNAs were found in all tissues and were particularly abundant in testis. At least three splice variants were found in the ORF and three variants in the 5'UTR.
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http://dx.doi.org/10.1006/bbrc.2002.6458 | DOI Listing |
Sci Data
December 2024
State Key Laboratory of Wheat Improvement, Peking University Institute of Advanced Agricultural Sciences, Shandong Laboratory of Advanced Agriculture Sciences in Weifang, Weifang, 261325, Shandong, China.
Wild relatives of wheat are valuable sources for enhancing the genetic diversity of common wheat. Aegilops comosa, an annual diploid species with an MM genome constitution, possesses numerous agronomically valuable traits that can be exploited for wheat improvement. In this study, we report a chromosome-level genome assembly of Ae.
View Article and Find Full Text PDFJ Phycol
December 2024
Faculty of Science, Department of Botany, University of South Bohemia, České Budějovice, Czech Republic.
Over 400 cyanobacterial genera have been described up to the present. Since the Cambridge Rules (https://www.iapt-taxon.
View Article and Find Full Text PDFEnzyme Microb Technol
December 2024
State Key Laboratory of Food Science and Resources, School of Food Science and Technology, Nanchang University, Nanchang 330047, China.
The exorbitant production costs associated with natural tannases pose a significant challenge to their widespread industrial utilization. Microbial expression systems provide a cost-effective method for enzyme production. In this study, a putative gene encoding the subtype B tannase (Gt-Tan) was cloned from Galactobacillus timonensis and expressed heterologously in Escherichia coli BL21 (DE3) cells.
View Article and Find Full Text PDFMethods Mol Biol
December 2024
School of Biological Sciences, University of Canterbury, Christchurch, New Zealand.
Yeast two-hybrid library screening enables the discovery of novel protein-protein interactions. Identifying cytosolic host proteins targeted by host-translocated Phytophthora effector proteins relies on the mRNA amount, quality, and composition used to prepare the yeast two-hybrid cDNA library. Here we describe the steps required for the preparation of a Pinus radiata cDNA library optimized for Phytophthora effector target screening in yeast.
View Article and Find Full Text PDFCurr Issues Mol Biol
December 2024
Department of Fisheries Science, Chonnam National University, Yeosu 59626, Republic of Korea.
Myogenic regulator factors (MRFs) are essential for skeletal muscle development in vertebrates, including fish. This study aimed to characterize the role of () in muscle development in Nile tilapia by cloning from muscle tissues. To explore the function of , CRISPR/Cas9 gene editing was employed.
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