AI Article Synopsis
- The study analyzed genetic variation of the H-FABP gene in 561 pigs from various breeds using PCR-RFLP.
- Nanchang white pigs-only showed the HH genotype, while other breeds exhibited variations at the Hinf I-RFLP site.
- Exotic breeds like Duroc and native breeds demonstrated polymorphism, but Chinese local breeds showed no variation at the Hae III-RFLP site, and only Duroc had variation at the Msp I-RFLP site, with local breeds identified as AADD-genotypes.
Article Abstract
The genetic variation of heart fatty acid-binding protein (H-FABP) gene in 561 pigs including Duroc, Landrace, Large Yorkshire, Nanchang white pig, Erhualian, Meishan, Yushan black pig, Leping spotted pig, Jinhua black head-hind pig and Shanggao black head-hind pig were detected by PCR-RFLP with Hinf I, Hae III and Msp I. The results showed as follows: (1) Nanchang white pig presented only HH genotype while other breeds varied at the Hinf I-RFLP site; (2) The exotic breeds including Duroc, Landrace, Large Yorkshire and the native breed Nanchang white pig were proved to be polymorphic while the five Chinese local breeds presented no variation at the Hae III-RFLP site; (3) Among the tested breeds only Duroc presented variation at the Msp I-RFLP site. It is noted that all the Chinese local breeds present as AADD--genotypes.
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