AI Article Synopsis
- Our understanding of how embryos move through the fallopian tubes is limited, but this study highlights the importance of molecules on the zona pellucida (ZP) in mouse embryos' transport.
- Interspecifically transferred embryos from bovine and porcine species tended to get stuck in the mouse oviduct rather than moving to the uterus, suggesting species compatibility affects transport.
- Treatments with specific antisera and enzymes showed that altering the ZP molecules significantly disrupted the transport process, leading to the hypothesis that unique ZP structures may interact with cilia or epithelial cells in the oviduct to facilitate transport.
Article Abstract
Our understandings of the molecular and cellular mechanisms underlying tubal transport of embryos are poor. This study describes the essential role of the molecules on the zona pellucida (ZP) in the tubal transport of mouse embryos. The bovine and porcine embryos that were interspecifically transferred to the mouse oviduct were selectively retained in the oviduct and rarely transported to the uterus. Antiserum ZP3-9 against synthetic peptides that are specific for mouse ZP3, significantly interfered with tubal transport of the treated embryos. The treatment of mouse embryos with antiserum ZP2-20 against the synthetic peptides, deduced from the sequences that are conserved in the structure of ZP2 from mouse and human, also inhibited their tubal transport. Among various proteolytic and glycosidic enzymes, treatments with trypsin and beta-glucosidase prior to transfer to the oviduct, significantly interfered with the tubal transport of the enzyme-treated mouse embryos. We hypothesize that species-specific epitopes on the ZP may be recognized by the oviductal cilia and/or the epithelial cells of ducts for tubal transport.
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