A protocol for quantification of human immunodeficiency virus type 1 (HIV-1) proviral DNA with the TaqMan technology was developed and validated. The assay was specific for HIV-1, with an analytic sensitivity of 10 copies and a linear dynamic range of >6 logs. Viral RNA levels, when at a stable state, were highly correlated with proviral DNA levels in 80 specimens of 18 HIV-infected children.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC153359 | PMC |
| http://dx.doi.org/10.1128/JCM.40.2.675-678.2002 | DOI Listing |
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